| Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans. | |
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MedLine Citation:
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PMID: 16511169 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 A, and diffract to 2.2 A resolution using synchrotron radiation. |
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Authors:
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Catherine Tricot; Sebastien van Aelst; Ruddy Wattiez; Max Mergeay; Victor Stalon; Johan Wouters |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-08-31 |
Journal Detail:
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Title: Acta crystallographica. Section F, Structural biology and crystallization communications Volume: 61 ISSN: 1744-3091 ISO Abbreviation: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2006-03-02 Completed Date: 2006-08-18 Revised Date: 2013-06-07 |
Medline Journal Info:
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Nlm Unique ID: 101226117 Medline TA: Acta Crystallogr Sect F Struct Biol Cryst Commun Country: England |
Other Details:
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Languages: eng Pagination: 825-7 Citation Subset: IM |
Affiliation:
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Institut de Recherches Microbiologiques JM Wiame, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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chemistry*,
isolation & purification* Burkholderiaceae / chemistry* Crystallization Crystallography, X-Ray Electrophoresis, Polyacrylamide Gel Gene Expression Molecular Structure |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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