| Overexpression of prefoldin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3 endowed Escherichia coli with organic solvent tolerance. | |
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MedLine Citation:
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PMID: 18443786 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Prefoldin is a jellyfish-shaped hexameric chaperone that captures a protein-folding intermediate and transfers it to the group II chaperonin for correct folding. In this work, we characterized the organic solvent tolerance of Escherichia coli cells that overexpress prefoldin and group II chaperonin from a hyperthermophilic archeaum, Pyrococcus horikoshii OT3. The colony-forming efficiency of E. coli cells overexpressing prefoldin increased by 1,000-fold and decreased the accumulation of intracellular organic solvent. The effect was impaired by deletions of the region responsible for the chaperone function of prefoldin. Therefore, we concluded that prefoldin endows E. coli cells by preventing accumulation of intracellular organic solvent through its molecular chaperone activity. |
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Authors:
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Mina Okochi; Kei Kanie; Masaki Kurimoto; Masafumi Yohda; Hiroyuki Honda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-04-29 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 79 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-19 Completed Date: 2008-09-09 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 443-9 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan. okochi@nubio.nagoya-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Archaeal Proteins
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chemistry,
genetics,
metabolism* Chaperonins / chemistry, genetics, metabolism Escherichia coli / drug effects*, genetics, growth & development*, metabolism Gene Expression* Hot Temperature Molecular Chaperones / chemistry, genetics, metabolism* Organic Chemicals / metabolism, pharmacology* Protein Folding Pyrococcus horikoshii / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Archaeal Proteins; 0/Molecular Chaperones; 0/Organic Chemicals; 0/prefoldin; EC 3.6.1.-/Chaperonins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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