Document Detail

Overexpression of the polycythemia rubra vera-1 gene in essential thrombocythemia.
MedLine Citation:
PMID:  12377969     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To examine the utility of polycythemia rubra vera-1 (PRV-1)-specific reverse transcriptase polymerase chain reaction (RT-PCR) to discriminate essential thrombocythemia (ET) and polycythemia vera (PV) from secondary thrombocytosis (ST) or secondary erythrocytosis (SE). PATIENTS AND METHODS: We analyzed the expression of PRV-1 in granulocytes isolated from 37 patients with ET, 37 patients with PV, 25 patients with ST, 10 patients with SE, 25 patients with secondary leukocytosis (SL), five patients with chronic myelogenous leukemia (CML), five patients with chronic idiopathic myelofibrosis (IM), five patients with myelodysplastic syndrome (MDS), and 20 normal individuals by PRV-1-specific RT-PCR. In female patients, PRV-1 expression was correlated with clonality analysis as assessed by the human androgen receptor polymorphism assay. RESULTS: PRV-1 was not expressed in granulocytes isolated from normal individuals or from patients with ST, SE, CML, IM, MDS, and inflammatory/infectious SL. On the contrary, all ET patients, 35 of 37 PV patients, and five patients with acute postsurgery and posttraumatic SL overexpressed PRV-1. All the cases with monoclonal hematopoiesis (17 of 21 with ET and 12 of 12 with PV) expressed PRV-1, yet PRV-1 overexpression extended also over the cases of ET showing polyclonal hematopoiesis (four of 20). CONCLUSION: The overexpression of PRV-1 seems to be a useful tool for discriminating ET and PV from ST and SE, thus offering an innovative diagnostic approach on the basis of the detection of positive diagnostic criteria instead of exclusion criteria.
Luciana Teofili; Maurizio Martini; Myriam Luongo; Antonella Di Mario; Giuseppe Leone; Valerio De Stefano; Luigi Maria Larocca
Related Documents :
17342649 - Neuraminidase inhibitors improve outcome of patients with leukemia and influenza: an ob...
8366299 - Usefulness of terminal deoxynucleotidyl transferase as prognosticator in leukemia patie...
3468999 - Differential pattern of oncogene and beta-actin expression in leukaemic cells from aml ...
3476149 - Ph-negative chronic myeloid leukaemia.
3166449 - Prediction of impending blast cell transformation in chronic granulocytic leukaemia.
12525519 - Additional prognostic value of bone marrow histology in patients subclassified accordin...
16330869 - Combined carotid and transcranial ultrasound findings compared with clinical classifica...
10669669 - Left ventricular thrombus and subsequent thromboembolism in patients with severe systol...
6695819 - Flecainide dose-response relations in stable ventricular arrhythmias.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical oncology : official journal of the American Society of Clinical Oncology     Volume:  20     ISSN:  0732-183X     ISO Abbreviation:  J. Clin. Oncol.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-14     Completed Date:  2002-10-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8309333     Medline TA:  J Clin Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4249-54     Citation Subset:  IM    
Istituto di Ematologia and Istituto di Anatomia Patologica, Università Cattolica del Sacro Cuore, Rome, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Aged, 80 and over
Gene Expression*
Granulocytes / metabolism
Hematopoiesis / genetics
Membrane Glycoproteins
Middle Aged
Polycythemia / diagnosis,  genetics
Polycythemia Vera / diagnosis,  genetics
Polymorphism, Genetic
Receptors, Androgen
Receptors, Cell Surface / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Thrombocythemia, Essential / diagnosis*,  genetics*
Thrombocytosis / diagnosis,  genetics
Reg. No./Substance:
0/AR protein, human; 0/CD177 protein, human; 0/Isoantigens; 0/Membrane Glycoproteins; 0/Receptors, Androgen; 0/Receptors, Cell Surface

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Chronic arm morbidity after curative breast cancer treatment: prevalence and impact on quality of li...
Next Document:  Pyothorax-associated lymphoma: a review of 106 cases.