Document Detail

Overexpression of p73 enhances cisplatin-induced apoptosis in HeLa cells.
MedLine Citation:
PMID:  16526280     Owner:  NLM     Status:  MEDLINE    
To examine a possible synergistic role for p73 and cisplatin (cis-diamminedichloroplatinum II) in HeLa cells with a nonfunctional p53 protein, we established stable HeLa/p73 clones using a tetracycline inducible eukaryotic expression vector. The HeLa/p73 clones were not characterized by changes in growth or morphology. Cell death analysis, however, indicated a greater sensitivity to cisplatin in the p73-overexpressed HeLa cells than determined for the non-induced HeLa cells. This increased sensitivity seems to affect an induction of a sub-G1 population as assessed from flow cytometry analysis. The increased sub-G1 population may, in turn, result from a reduction of cyclin D1 and B1 expression by cisplatin in the presence of p73. Hoechest staining indicated an increased number of dead cells in the p73-induced cells compared to the non-induced cells. Poly ADP-ribose polymerase (PARP) cleavage was shown to be distinct in the p73-overexpressed cells compared to non-induced cells, which suggests that p73 modulates the cisplatin-induced apoptosis. Therefore, a synergistic effect of p73 and cisplatin to induce apoptosis could lead to new treatment for some types of human cancers.
Keun-Cheol Kim; Chul-Soo Jung; Kyung-Hee Choi
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Archives of pharmacal research     Volume:  29     ISSN:  0253-6269     ISO Abbreviation:  Arch. Pharm. Res.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-03-10     Completed Date:  2006-06-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8000036     Medline TA:  Arch Pharm Res     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  152-8     Citation Subset:  IM    
Division of Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, Korea.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects*
Cisplatin / pharmacology*
Cyclin B / metabolism
Cyclin B1
Cyclin D1 / metabolism
DNA-Binding Proteins / genetics,  metabolism*
Dose-Response Relationship, Drug
Genes, Tumor Suppressor
Hela Cells / drug effects*,  metabolism
Nuclear Proteins / genetics,  metabolism*
Tumor Suppressor Proteins
Reg. No./Substance:
0/Antineoplastic Agents; 0/CCNB1 protein, human; 0/Cyclin B; 0/Cyclin B1; 0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/Tumor Suppressor Proteins; 0/tumor suppressor protein p73; 136601-57-5/Cyclin D1; 15663-27-1/Cisplatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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