| Overexpression of p73 enhances cisplatin-induced apoptosis in HeLa cells. | |
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MedLine Citation:
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PMID: 16526280 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To examine a possible synergistic role for p73 and cisplatin (cis-diamminedichloroplatinum II) in HeLa cells with a nonfunctional p53 protein, we established stable HeLa/p73 clones using a tetracycline inducible eukaryotic expression vector. The HeLa/p73 clones were not characterized by changes in growth or morphology. Cell death analysis, however, indicated a greater sensitivity to cisplatin in the p73-overexpressed HeLa cells than determined for the non-induced HeLa cells. This increased sensitivity seems to affect an induction of a sub-G1 population as assessed from flow cytometry analysis. The increased sub-G1 population may, in turn, result from a reduction of cyclin D1 and B1 expression by cisplatin in the presence of p73. Hoechest staining indicated an increased number of dead cells in the p73-induced cells compared to the non-induced cells. Poly ADP-ribose polymerase (PARP) cleavage was shown to be distinct in the p73-overexpressed cells compared to non-induced cells, which suggests that p73 modulates the cisplatin-induced apoptosis. Therefore, a synergistic effect of p73 and cisplatin to induce apoptosis could lead to new treatment for some types of human cancers. |
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Authors:
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Keun-Cheol Kim; Chul-Soo Jung; Kyung-Hee Choi |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Archives of pharmacal research Volume: 29 ISSN: 0253-6269 ISO Abbreviation: Arch. Pharm. Res. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-03-10 Completed Date: 2006-06-21 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8000036 Medline TA: Arch Pharm Res Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 152-8 Citation Subset: IM |
Affiliation:
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Division of Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, Korea. kckim@kangwon.ac.kr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology* Apoptosis / drug effects* Cisplatin / pharmacology* Cyclin B / metabolism Cyclin B1 Cyclin D1 / metabolism DNA-Binding Proteins / genetics, metabolism* Dose-Response Relationship, Drug Genes, Tumor Suppressor Hela Cells / drug effects*, metabolism Humans Nuclear Proteins / genetics, metabolism* Transfection Tumor Suppressor Proteins |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/CCNB1 protein, human; 0/Cyclin B; 0/Cyclin B1; 0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/Tumor Suppressor Proteins; 0/tumor suppressor protein p73; 136601-57-5/Cyclin D1; 15663-27-1/Cisplatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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