Document Detail

Overexpression of p150, a part of the large subunit of the eukaryotic translation initiation factor 3, in colon cancer.
MedLine Citation:
PMID:  20530408     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: P150, a 150 kDa protein, was isolated from virally and oncogene-transformed mouse cell lines, partially purified and cloned. P150 is part of the large subunit of the eukaryotic translation initiation factor 3 with sequence homology to centrosomin A. A significant correlation between p150 expression and malignancy in breast, cervical and esophageal cancer have recently been demonstrated. MATERIALS AND METHODS: Here, 110 colorectal carcinomas of different grades and stages, including lymph node and liver metastases were compared to adjacent normal mucosa by immunohistochemistry of P150. Western blot analysis of selected cases confirmed the expression levels determined by immunohistochemistry. Additionally, immuno-electron and laser scanning microscopy (LSM) was performed. RESULTS: All investigated carcinomas revealed high levels of p150 protein compared to normal adjacent mucosa. The staining intensity was slightly heterogeneous, and positivity was correlated to the tumor grade with statistically significant differences of p150 expression between normal and neoplastic mucosa (p<0.0001, Kruskal-Wallis test). Western blots confirmed higher expression levels of p150 in the tumor. Immunogold labelling and LSM investigation showed high expression levels of p150 on the rough endoplasmic reticulum and polyribosomes, indicating that p150 is translationally active in these tumors. CONCLUSION: Thus, we propose that p150 plays an important role in development and growth of colorectal carcinomas. Furthermore, p150 expression might provide us with reliable information on the biological behaviour of tumors and the clinical course of the disease.
J Haybaeck; T O'Connor; R Spilka; G Spizzo; Ch Ensinger; G Mikuz; T Brunhuber; A Vogetseder; I Theurl; W Salvenmoser; H Draxl; R Bänziger; F Bachmann; G Schäfer; M Burger; P Obrist
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-06-09     Completed Date:  2010-06-30     Revised Date:  2010-09-17    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1047-55     Citation Subset:  IM    
Institute of Neuropathology, Department of Pathology, University Hospital Zurich, Switzerland.
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MeSH Terms
Cell Differentiation / physiology
Colorectal Neoplasms / metabolism*,  pathology
Eukaryotic Initiation Factor-3 / biosynthesis*
Microscopy, Confocal
Microscopy, Electron
Middle Aged
Neoplasm Staging
Reg. No./Substance:
0/EIF3A protein, human; 0/Eukaryotic Initiation Factor-3

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