Document Detail

Overexpression of a novel osteopetrosis-related gene CCDC154 suppresses cell proliferation by inducing G2/M arrest.
MedLine Citation:
PMID:  22895184     Owner:  NLM     Status:  MEDLINE    
Osteopetrosis, a disorder of skeletal bone, can cause death during childhood. We previously described a new spontaneous autosomal recessive osteopetrosis mouse mutant, "new toothless" (ntl). In this study, we reported for the first time the identification, cloning and characterization of the coiled-coil domain-containing 154 (CCDC154), a novel gene whose deletion of ~5 kb sequence including exons 1-6 was completely linked to the ntl mutant. The CCDC154 was conserved between mouse and human and is wildly expressed in mouse tissues. The cellular localization of CCDC154 was in the early endosomes. Overexpression of CCDC154 inhibited cell proliferation of HEK293 cells by inducing G 2/M arrest. CCDC154 also inhibited tumor cell growth, and the soft agar assay revealed a significant decrease of the colony size of Hela cells upon transfection of CCDC154. Our results indicate that CCDC154 is a novel osteopetrosis-related gene involved in cell cycle regulation and tumor suppression growth.
Wanqin Liao; Rongsen Zhao; Liting Lu; Rongrong Zhang; Jiawei Zou; Tao Xu; Changjie Wu; Jiajia Tang; Yuezhen Deng; Xincheng Lu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-16
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  11     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-13     Completed Date:  2013-02-04     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3270-9     Citation Subset:  IM    
Institute of Genomic Medicine, Wenzhou Medical College, Wenzhou, China.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cell Cycle Proteins / chemistry,  genetics,  metabolism*
Cell Proliferation
DNA Primers / genetics
Endosomes / metabolism
G2 Phase Cell Cycle Checkpoints / genetics,  physiology*
HEK293 Cells
HeLa Cells
Microscopy, Fluorescence
Models, Molecular*
Molecular Sequence Data
Osteopetrosis / genetics,  metabolism*
Polymerase Chain Reaction
Sequence Analysis, DNA
Tumor Suppressor Proteins / chemistry,  genetics,  metabolism*
Reg. No./Substance:
0/CCDC154 protein, human; 0/Cell Cycle Proteins; 0/DNA Primers; 0/Tumor Suppressor Proteins

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