| Overexpression of microRNA-145 in atherosclerotic plaques from hypertensive patients. | |
| | |
MedLine Citation:
|
PMID: 23339529 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Background: MicroRNAs (miRNAs) are endogenous, non-coding, short, single-stranded RNAs and represent a new class of gene regulators. Recent evidence supports a role for miRNAs in cardiovascular pathophysiology and atherosclerosis development. We have previously demonstrated that miR-145 is widely expressed in human atherosclerotic lesions and its downregulation has been correlated with vascular smooth muscle cell dedifferentiation, a cardinal step in the development of atherosclerosis. However, no evidences are available at this time about modulation of miR-145 in the setting of hypertension. Thus, the aim of this study was to investigate the expression of miR-145 in complicated hypertension. Materials and methods: Atherosclerotic plaques were obtained from 22 patients undergoing carotid endarterectomy for high-grade internal carotid artery stenosis. Plaques were subdivided into hypertension (n = 15) and control (n = 7) groups according to the presence or absence of hypertension (as defined by blood pressure > 140/90 mmHg or current antihypertensive treatment). In study plaques, miR-145 values were evaluated using real-time PCR. The level of induction has been tested by using ΔΔ cycle threshold method. Results: We found that miR-145 was significantly more expressed in atherosclerotic plaques of hypertensive patients than in control plaques (1.201 ± 0.260 vs 0.483 ± 0.148 fold induction ± SE; p = 0.026). Moreover, a post-hoc analysis showed that treatment with angiotensin receptor blockers may be associated with the maximum increase in miR-145 levels, although these data did not show any statistical significance probably due to the limited sample size. Conclusions: To the best of our knowledge, this study is the first demonstration that hypertension may upregulate miR-145 expression in human atherosclerotic plaques. Future investigations will be necessary to establish the molecular readout of miR-145 upregulation in atherosclerotic lesions in hypertension. |
| | |
Authors:
|
Donato Santovito; Claudia Mandolini; Pamela Marcantonio; Velia De Nardis; Marco Bucci; Camilla Paganelli; Francesco Magnacca; Sante Ucchino; Daniela Mastroiacovo; Giovambattista Desideri; Andrea Mezzetti; Francesco Cipollone |
Related Documents
:
|
1009279 - Detection and treatment of hypertension in an inner london community. 1554809 - Assessing the validity of childhood blood pressure screening: unbiased estimates of sen... 17257789 - Manufacture of fine spherical granules by an extrusion/spheronization method. 10972219 - Intragraft expression of transforming growth factor-beta 1 by a novel quantitative reve... 7025369 - Effect of hypotension on the results of kidney storage and the use of dopamine under th... 23458589 - Oscillometric carotid to femoral pulse wave velocity estimated with the vicorder device. 9118169 - Nisoldipine cc: clinical experience in hypertension. 2520939 - Diagnosis of myocardial ischemia by the pressure-rate quotient and diastolic time inter... 9283619 - Continuous wavelet transform of aortic pressure oscillations in anesthetized dogs: effe... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-23 |
Journal Detail:
|
Title: Expert opinion on therapeutic targets Volume: - ISSN: 1744-7631 ISO Abbreviation: Expert Opin. Ther. Targets Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101127833 Medline TA: Expert Opin Ther Targets Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
'G. d'Annunzio' University, European Center of Excellence on Atherosclerosis, Hypertension and Dyslipidemia, and Clinical Research Center, Center of Excellence on Aging (Ce.S.I.) , Via dei Vestini, 66, 66100 Chieti (CH) , Italy +39 0871 357733 ; + 39 0871 357733 ; fcipollone@unich.it. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Spectral analysis of word-initial alveolar and velar plosives produced by Iranian children with clef...
Next Document: Pharmacases.de - A student-centered e-learning project of clinical pharmacology.