Document Detail


Overexpression of microRNA-145 in atherosclerotic plaques from hypertensive patients.
MedLine Citation:
PMID:  23339529     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background: MicroRNAs (miRNAs) are endogenous, non-coding, short, single-stranded RNAs and represent a new class of gene regulators. Recent evidence supports a role for miRNAs in cardiovascular pathophysiology and atherosclerosis development. We have previously demonstrated that miR-145 is widely expressed in human atherosclerotic lesions and its downregulation has been correlated with vascular smooth muscle cell dedifferentiation, a cardinal step in the development of atherosclerosis. However, no evidences are available at this time about modulation of miR-145 in the setting of hypertension. Thus, the aim of this study was to investigate the expression of miR-145 in complicated hypertension. Materials and methods: Atherosclerotic plaques were obtained from 22 patients undergoing carotid endarterectomy for high-grade internal carotid artery stenosis. Plaques were subdivided into hypertension (n = 15) and control (n = 7) groups according to the presence or absence of hypertension (as defined by blood pressure > 140/90 mmHg or current antihypertensive treatment). In study plaques, miR-145 values were evaluated using real-time PCR. The level of induction has been tested by using ΔΔ cycle threshold method. Results: We found that miR-145 was significantly more expressed in atherosclerotic plaques of hypertensive patients than in control plaques (1.201 ± 0.260 vs 0.483 ± 0.148 fold induction ± SE; p = 0.026). Moreover, a post-hoc analysis showed that treatment with angiotensin receptor blockers may be associated with the maximum increase in miR-145 levels, although these data did not show any statistical significance probably due to the limited sample size. Conclusions: To the best of our knowledge, this study is the first demonstration that hypertension may upregulate miR-145 expression in human atherosclerotic plaques. Future investigations will be necessary to establish the molecular readout of miR-145 upregulation in atherosclerotic lesions in hypertension.
Authors:
Donato Santovito; Claudia Mandolini; Pamela Marcantonio; Velia De Nardis; Marco Bucci; Camilla Paganelli; Francesco Magnacca; Sante Ucchino; Daniela Mastroiacovo; Giovambattista Desideri; Andrea Mezzetti; Francesco Cipollone
Related Documents :
1009279 - Detection and treatment of hypertension in an inner london community.
1554809 - Assessing the validity of childhood blood pressure screening: unbiased estimates of sen...
17257789 - Manufacture of fine spherical granules by an extrusion/spheronization method.
10972219 - Intragraft expression of transforming growth factor-beta 1 by a novel quantitative reve...
7025369 - Effect of hypotension on the results of kidney storage and the use of dopamine under th...
23458589 - Oscillometric carotid to femoral pulse wave velocity estimated with the vicorder device.
9118169 - Nisoldipine cc: clinical experience in hypertension.
2520939 - Diagnosis of myocardial ischemia by the pressure-rate quotient and diastolic time inter...
9283619 - Continuous wavelet transform of aortic pressure oscillations in anesthetized dogs: effe...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-23
Journal Detail:
Title:  Expert opinion on therapeutic targets     Volume:  -     ISSN:  1744-7631     ISO Abbreviation:  Expert Opin. Ther. Targets     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101127833     Medline TA:  Expert Opin Ther Targets     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
'G. d'Annunzio' University, European Center of Excellence on Atherosclerosis, Hypertension and Dyslipidemia, and Clinical Research Center, Center of Excellence on Aging (Ce.S.I.) , Via dei Vestini, 66, 66100 Chieti (CH) , Italy +39 0871 357733 ; + 39 0871 357733 ; fcipollone@unich.it.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Spectral analysis of word-initial alveolar and velar plosives produced by Iranian children with clef...
Next Document:  Pharmacases.de - A student-centered e-learning project of clinical pharmacology.