| Overexpression of the inhibitor protein IF(1) in AS-30D hepatoma produces a higher association with mitochondrial F(1)F(0) ATP synthase compared to normal rat liver: functional and cross-linking studies. | |
| | |
MedLine Citation:
|
PMID: 15337856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
According to functional studies, the higher IF(1) content reported in mitochondria of cancerous cells is supposed to induce a higher association with the F(1)F(0) complex than in normal cells and therefore a better inhibition of its ATPase activity. The first structural evidence supporting this prediction is here presented. Densitometric analyses of Western blotting experiments indicated a 2-fold increase in IF(1) content of AS-30D submitochondrial particles compared to normal rat liver controls. The ratio of IF(1)/F(1) alpha subunit increased similarly as judged by Westernblot analyses. This IF(1) overexpression correlated with a slower rate of IF(1) release (F(1)F(0)-ATPase activation) from the F(1)F(0) complex in AS-30D than in normal rat liver submitochondrial particles. The IF(1)-IF(1), gamma-IF(1), and alpha-IF(1) cross-linkages previously formed with dithiobis(succinimidylpropionate) in bovine F(1)F(0)I and IF(1) complexes were reproduced in the F(1)F(0)I-ATP synthase of hepatoma AS-30D cells. However, a much lower yield of IF(1) cross-linkages was found in normal rat liver particles which made them almost undetectable in SMP as well as in the immunoprecipitated F(1)F(0)I complex. Modeling in vivo IF(1) overexpression of cancerous cells by in vitro reconstitution of excess recombinant IF(1) with rat liver submitochondrial particles devoid of IF(1) reproduced the same IF(1) cross-linkages observed in AS-30D particles. |
| | |
Authors:
|
Concepción Bravo; Fernando Minauro-Sanmiguel; Edgar Morales-Ríos; José S Rodríguez-Zavala; José J García |
Related Documents
:
|
2986556 - Superoxide-dependent lipid peroxidation and vitamin e content of microsomes from hepato... 9789686 - Wistar furth rat megakaryocytes lack dense compartments and intercellular plaques, memb... 206706 - Gangliosides of liver tumors induced by n-2-fluorenylacetamide. i. ganglioside alterati... 6131386 - The particular traits of carcinogenesis induced in wistar rats by aflatoxin b1. iv. por... 1779226 - Sexually dimorphic, androgen sensitive, enkephalinergic afferents to a lumbar motor nuc... 9754056 - Loss of a putative novel growth inhibitory apoptotic 14 kd polypeptide during progressi... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of bioenergetics and biomembranes Volume: 36 ISSN: 0145-479X ISO Abbreviation: J. Bioenerg. Biomembr. Publication Date: 2004 Jun |
Date Detail:
|
Created Date: 2004-08-31 Completed Date: 2005-03-07 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7701859 Medline TA: J Bioenerg Biomembr Country: United States |
Other Details:
|
Languages: eng Pagination: 257-64 Citation Subset: IM |
Affiliation:
|
Departamento de Bioquímica, Instituto Nacional de Cardiología, "Ignacio Chávez," México, México. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blotting, Western Cross-Linking Reagents / metabolism* Densitometry Electrophoresis, Polyacrylamide Gel Enzyme Inhibitors / metabolism* Liver Neoplasms, Experimental / metabolism* Mitochondria / metabolism* Mitochondrial Proton-Translocating ATPases / metabolism* Oligonucleotides Plasmids / genetics Rats |
| Chemical | |
Reg. No./Substance:
|
0/Cross-Linking Reagents; 0/Enzyme Inhibitors; 0/Oligonucleotides; EC 3.6.1.-/F1F0-ATP synthase; EC 3.6.3.-/Mitochondrial Proton-Translocating ATPases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ligand-dependent structural changes in the V(1) ATPase from Manduca sexta.
Next Document: Functional approach to the catalytic site of the sarcoplasmic reticulum Ca(2+)-ATPase: binding and h...