Document Detail

Overexpression of the growth arrest-specific homeobox gene Gax inhibits proliferation, migration, cell cycle progression, and apoptosis in serum-induced vascular smooth muscle cells.
MedLine Citation:
PMID:  24737425     Owner:  NLM     Status:  In-Data-Review    
The Gax gene has been implicated in a variety of cell-developmental and biological processes, and aberrant Gax expression is linked to many diseases. In this study, to provide important insights for Gax-based gene therapy in vein graft restenosis and its anti-restenotic mechanism, we used rabbit vascular smooth muscle cells (VSMCs) to investigate the effects of Gax overexpression on proliferation, migration, cell cycle, and apoptosis in a serum-stimulated culture. Rabbit VSMC lines that stably overexpressed Gax were established by transfection with recombinant adenoviral vector Ad5-Gax. The effect of Gax overexpression on in vitro serum-induced VSMCs proliferation, migration, cell cycle, and apoptosis was assessed by MTT, wound healing, and flow cytometry assays, respectively. To investigate the effect of Gax overexpression on PCNA and MMP-2 in serum-induced VSMCs, immunocytochemistry, RT-PCR, and gelatin zymography were performed. The results clearly showed that Gax overexpression decreases PCNA expression in serum-induced VSMCs. Gax overexpression also significantly inhibited cell proliferation by blocking entry into the S-phase of the cell cycle, promoted cell apoptosis, and reduced cell migration activity by downregulating MMP-2 release and activity. These findings indicate that Gax would be an optimal target gene for gene therapy to treat vein graft restenosis.
H Zheng; S Xue; Z L Hu; J G Shan; W G Yang
Related Documents :
24914695 - Intervertebral disc regeneration: do nutrients lead the way?
24021805 - Nongenomic, glucocorticoid receptor-mediated regulation of serotonin transporter cell s...
25132065 - Effect of lithium chloride on the production and sialylation of fc-fusion protein in ch...
24329605 - Proton pump inhibitor-induced clear cell change.
22138495 - Single cell raman spectroscopy for cell sorting and imaging.
25061855 - Conjunctival epithelial and goblet cell function in chronic inflammation and ocular all...
Publication Detail:
Type:  Journal Article     Date:  2014-03-24
Journal Detail:
Title:  Genetics and molecular research : GMR     Volume:  13     ISSN:  1676-5680     ISO Abbreviation:  Genet. Mol. Res.     Publication Date:  2014  
Date Detail:
Created Date:  2014-04-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101169387     Medline TA:  Genet Mol Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  1993-2008     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Short Communication Microsatellite markers derived from Japanese scallop (Mizuhopecten yessoensis) e...
Next Document:  Prediction of disease-related microRNAs by incorporating functional similarity and common associatio...