Document Detail

Overexpression of glutaredoxin protects cardiomyocytes against nitric oxide-induced apoptosis with suppressing the S-nitrosylation of proteins and nuclear translocation of GAPDH.
MedLine Citation:
PMID:  22846575     Owner:  NLM     Status:  Publisher    
There is increasing evidence demonstrating that glutaredoxin 1 (GRX1), a cytosolic enzyme responsible for the catalysis of protein deglutathionylation, plays distinct roles in inflammation and apoptosis by inducing changes in the cellular redox system. In this study, we investigated whether and how the overexpression of GRX1 protects cardiomyocytes against nitric oxide (NO)-induced apoptosis. Cardiomyocytes (H9c2 cells) were transfected with the expression vector for mouse GRX1 cDNA, and mock-transfected cells were used as a control. Compared with the mock-transfected cells, the GRX1-transfected cells were more resistant to NO-induced apoptosis. Stimulation with NO significantly increased the nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a pro-apoptotic protein, in the mock-transfected cells, but did not change GAPDH localization in the GRX1-transfected cells. Furthermore, we found that NO stimulation clearly induced the oxidative modification of GAPDH in the mock-transfected cells, whereas less modification of GAPDH was observed in the GRX1-transfected cells. These data suggest that the overexpression of GRX1 could protect cardiomyocytes against NO-induced apoptosis, likely through the inhibition of the oxidative modification and the nuclear translocation of GAPDH.
Chiaki Inadomi; Hiroaki Murata; Yoshito Ihara; Shinji Goto; Yoshishige Urata; Junji Yodoi; Takahito Kondo; Koji Sumikawa
Related Documents :
22328385 - Immune responses to candida albicans in models of in vitro reconstituted human oral epi...
22641025 - Mincle polarizes human monocyte and neutrophil responses to candida albicans.
22677045 - Il-17a is essential for cell activation and inflammatory gene circuits in subjects with...
22561065 - Immunomodulatory roles and functional analysis of pre-b lymphocyte dt40 cells with the ...
9665335 - Localization of oxytocin receptors in the human and macaque monkey male reproductive tr...
22191045 - Immunopathogenesis in chlamydia trachomatis infected women.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-27
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  -     ISSN:  1090-2104     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Department of Anesthesiology, Nagasaki University School of Medicine, Nagasaki, 852-8501, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Ischemic Preconditioning Enhances Integrity of Coronary Endothelial Tight Junctions.
Next Document:  Hydrogen Sulfide Prevents Hypoxia-induced Apoptosis via Inhibition of an H(2)O(2)-activated Calcium ...