| Overexpression of eNOS in NTS causes hypotension and bradycardia in vivo. | |
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MedLine Citation:
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PMID: 11116119 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The role of nitric oxide (NO) in the brain in the control of blood pressure and the sympathetic nervous system is debated. This study examined the effect of overexpression of endothelial NO synthase (eNOS) in the nucleus tractus solitarii (NTS) on blood pressure in conscious rats. Adenovirus vectors encoding either eNOS (AdeNOS) or ss-galactosidase were transfected into the NTS in vivo. In the AdeNOS-treated rats, the local expression of eNOS in the NTS was confirmed by immunohistochemical staining and Western blot analysis for the eNOS protein and by increased production of nitrite/nitrate in the NTS measured by in vivo microdialysis. Blood pressure and heart rate, monitored by the use of a radiotelemetry system in a conscious state, were significantly decreased in the AdeNOS-treated group at day 5 to day 10 after the gene transfer. Urinary norepinephrine excretion also was decreased at day 7 after the gene transfer in the AdeNOS-treated group. Our results indicate that overexpression of eNOS in the NTS decreases blood pressure, heart rate, and sympathetic nerve activity in conscious rats. |
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Authors:
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K Sakai; Y Hirooka; I Matsuo; K Eshima; H Shigematsu; H Shimokawa; A Takeshita |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hypertension Volume: 36 ISSN: 1524-4563 ISO Abbreviation: Hypertension Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2000-12-26 Completed Date: 2001-01-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7906255 Medline TA: Hypertension Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1023-8 Citation Subset: IM |
Affiliation:
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Department of Cardiovascular Medicine, Cardiovascular Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Pressure / physiology* Bradycardia / enzymology Gene Expression Heart Rate Hypotension / enzymology Male Nitric Oxide / biosynthesis, physiology* Nitric Oxide Synthase / genetics, physiology* Nitric Oxide Synthase Type III Rats Rats, Inbred WKY Solitary Nucleus / enzymology, physiology* Transfection beta-Galactosidase / analysis, genetics, physiology |
| Chemical | |
Reg. No./Substance:
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10102-43-9/Nitric Oxide; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos3 protein, rat; EC 3.2.1.23/beta-Galactosidase |
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