Document Detail

Overexpression of eNOS in NTS causes hypotension and bradycardia in vivo.
MedLine Citation:
PMID:  11116119     Owner:  NLM     Status:  MEDLINE    
The role of nitric oxide (NO) in the brain in the control of blood pressure and the sympathetic nervous system is debated. This study examined the effect of overexpression of endothelial NO synthase (eNOS) in the nucleus tractus solitarii (NTS) on blood pressure in conscious rats. Adenovirus vectors encoding either eNOS (AdeNOS) or ss-galactosidase were transfected into the NTS in vivo. In the AdeNOS-treated rats, the local expression of eNOS in the NTS was confirmed by immunohistochemical staining and Western blot analysis for the eNOS protein and by increased production of nitrite/nitrate in the NTS measured by in vivo microdialysis. Blood pressure and heart rate, monitored by the use of a radiotelemetry system in a conscious state, were significantly decreased in the AdeNOS-treated group at day 5 to day 10 after the gene transfer. Urinary norepinephrine excretion also was decreased at day 7 after the gene transfer in the AdeNOS-treated group. Our results indicate that overexpression of eNOS in the NTS decreases blood pressure, heart rate, and sympathetic nerve activity in conscious rats.
K Sakai; Y Hirooka; I Matsuo; K Eshima; H Shigematsu; H Shimokawa; A Takeshita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hypertension     Volume:  36     ISSN:  1524-4563     ISO Abbreviation:  Hypertension     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2000-12-26     Completed Date:  2001-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1023-8     Citation Subset:  IM    
Department of Cardiovascular Medicine, Cardiovascular Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
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MeSH Terms
Blood Pressure / physiology*
Bradycardia / enzymology
Gene Expression
Heart Rate
Hypotension / enzymology
Nitric Oxide / biosynthesis,  physiology*
Nitric Oxide Synthase / genetics,  physiology*
Nitric Oxide Synthase Type III
Rats, Inbred WKY
Solitary Nucleus / enzymology,  physiology*
beta-Galactosidase / analysis,  genetics,  physiology
Reg. No./Substance:
10102-43-9/Nitric Oxide; EC Oxide Synthase; EC Oxide Synthase Type III; EC protein, rat; EC

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