| Overexpression of cyclin E does not influence homologous recombination in Chinese hamster cells. | |
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MedLine Citation:
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PMID: 12163026 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Overexpressed cyclin E in tumours is a prognosticator for poor patient outcome. Cells that overexpress cyclin E have been shown to be impaired in S-phase progression and exhibit genetic instability that may drive this subset of cancers. However, the origin for genetic instability caused by cyclin E overexpression is unknown. Homologous recombination plays an important role in S-phase progression and is also regulated by the same proteins that regulate cyclin E-associated kinase activity, i.e., p53 and p21. To test the hypothesis that overexpressed cyclin E causes genetic instability through homologous recombination, we investigated the effect of cyclin E overexpression on homologous recombination in the hprt gene in a Chinese hamster cell line. Although cyclin E overexpression shortened the G1 phase in the cell cycle as expected, we could see no change in neither spontaneous nor etoposide-induced recombination. Also, overexpression of cyclin E did not affect the repair of DNA double-strand breaks and failed to potentiate the cytotoxic effects of etoposide. Our data suggest that genetic instability caused by overexpression of cyclin E is not mediated by aberrant homologous recombination. |
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Authors:
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Cecilia Lundin; Magnus K R Samuelsson; Thomas Helleday |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 296 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-06 Completed Date: 2002-09-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 363-7 Citation Subset: IM |
Affiliation:
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Department of Genetic and Cellular Toxicology, Stockholm University, Stockholm, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals CHO Cells Cell Cycle* / drug effects Cricetinae Cyclin E / genetics, metabolism* DNA / metabolism DNA Damage Etoposide / pharmacology Flow Cytometry Humans Nucleic Acid Synthesis Inhibitors / pharmacology Recombination, Genetic* |
| Chemical | |
Reg. No./Substance:
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0/Cyclin E; 0/Nucleic Acid Synthesis Inhibitors; 33419-42-0/Etoposide; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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