Document Detail

Overexpression of cation-dependent mannose 6-phosphate receptor prevents cell death induced by serum deprivation in PC12 cells.
MedLine Citation:
PMID:  9790931     Owner:  NLM     Status:  MEDLINE    
PC12 cells express well cation-independent mannose 6-phosphate receptors (CI-MPR), but not cation-dependent (CD)-MPR as much. To examine CD-MPR dependency of transport of cathepsins B and D to lysosomes in PC12 cells, we prepared the cells overexpressing CD-MPR. Immunoreactivity for cathepsin B became more distinct and larger in size in the transfected cells than in wild-type cells. No difference in the distribution of cathepsin D was seen between these two cells. The viability of the cells following serum deprivation was significantly higher in the transfected cells than in wild-type cells. This increased viability of the transfected cells was blocked by CA074, a specific inhibitor of cathepsin B, while pepstatin A suppressed the action of CA074. The results suggest that CD-MPR preferentially transport cathepsin B in PC12 cells, and cathepsins B and D participate in the regulation of PC12 cell apoptosis.
S Kanamori; S Waguri; M Shibata; K Isahara; Y Ohsawa; A Konishi; S Kametaka; T Watanabe; S Ebisu; E Kominami; Y Uchiyama
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  251     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-11-23     Completed Date:  1998-11-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  204-8     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
Department of Conservative Dentistry, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan.
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MeSH Terms
Amino Acid Sequence
Apoptosis / physiology*
Cathepsin B / analysis
Cathepsin D / analysis
Cations / metabolism*
Cell Survival
Culture Media, Serum-Free / pharmacology
Molecular Sequence Data
PC12 Cells / metabolism*
Receptor, IGF Type 2 / biosynthesis*
Reg. No./Substance:
0/Cations; 0/Culture Media, Serum-Free; 0/Receptor, IGF Type 2; EC B; EC D

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