| Overexpression of catalase in cytosolic or mitochondrial compartment protects HepG2 cells against oxidative injury. | |
| | |
MedLine Citation:
|
PMID: 10473575 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
HepG2 cells were transfected with vectors containing human catalase cDNA and catalase cDNA with a mitochondrial leader sequence to allow comparison of the effectiveness of catalase overexpressed in the cytosolic or mitochondrial compartments to protect against oxidant-induced injury. Overexpression of catalase in cytosol and in mitochondria was confirmed by Western blot, and activity measurement and stable cell lines were established. The intracellular level of H(2)O(2) induced by exogenously added H(2)O(2) or antimycin A was lower in C33 cell lines overexpressing catalase in the cytosol and mC5 cell lines overexpressing catalase in the mitochondria as compared with Hp cell lines transfected with empty vector. Cell death caused by H(2)O(2), antimycin A, and menadione was considerably suppressed in both the mC5 and C33 cell lines. C33 and mC5 cells were also more resistant to apoptosis induced by H(2)O(2) and to the loss of mitochondrial membrane potential induced by H(2)O(2) and antimycin A. In view of the comparable protection by catalase overexpressed in the cytosol versus the mitochondria, catalase produced in both cellular compartments might act as a sink to decompose H(2)O(2) and move diffusable H(2)O(2) down its concentration gradient. The present study suggests that catalase in cytosol and catalase in mitochondria are capable of protecting HepG2 cells against cytotoxicity or apoptosis induced by oxidative stress. |
| | |
Authors:
|
J Bai; A M Rodriguez; J A Melendez; A I Cederbaum |
Related Documents
:
|
3785185 - Transcription and translation of mitochondrial dna in interspecific somatic cell hybrids. 15927145 - Synergistic effects of hydrogen peroxide and ethanol on cell viability loss in pc12 cel... 18068945 - Aloin induces apoptosis in jurkat cells. 20002195 - Atorvastatin-induced cell toxicity in yeast is linked to disruption of protein isopreny... 8355205 - Effect of emetine on t-2 toxin-induced inhibition of protein synthesis in mammalian cells. 21538935 - Epithelial membrane protein 1 inhibits human spinal chondrocyte differentiation. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 274 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1999 Sep |
Date Detail:
|
Created Date: 1999-10-07 Completed Date: 1999-10-07 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 26217-24 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, New York, New York 10029, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antimycin A
/
pharmacology Catalase / metabolism* Cytosol / enzymology* DNA Fragmentation Humans Hydrogen Peroxide / metabolism Membrane Potentials Mitochondria / enzymology*, physiology Oxidative Stress* Tumor Cells, Cultured Vitamin K / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
AA 03312/AA/NIAAA NIH HHS; CA 77068/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
12001-79-5/Vitamin K; 642-15-9/Antimycin A; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.6/Catalase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nerve growth factor (NGF)-induced calcium influx and intracellular calcium mobilization in 3T3 cells...
Next Document: Dissecting the role of a conserved motif (the second region of homology) in the AAA family of ATPase...