| Overexpression of bcl-2 protects hepatoma cell line HCC-9204 from ethanol-induced apoptosis. | |
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MedLine Citation:
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PMID: 11930666 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the effect of overexpression of bcl-2 on ethanol-induced apoptosis of primary hepatocellular carcinoma (HCC) cells. METHODS: The retrovirus expression vector pDOR-SB containing human bcl-2 cDNA was introduced into a human HCC cell line HCC-9204 by liposome-mediated transfection. pDOR-transfected and non-transfected HCC-9204 cells were used as controls. The expression of Bcl-2 protein by transfected HCC-9204 cells was detected by the immunohistochemical method. Then the cells were cloned with the limited dilution method continually until a monoclonal cell strain whose positive rate of Bcl-2 protein was 100% detected by flow cytometry was obtained. The killing rates of cells were detected by Methabenzthiazuron assay after the treatment of 6% ethanol for 6 h. The extent of apoptosis was analyzed by transferase-mediated dUTP nick end labeling (TUNEL) staining and flow cytometry. RESULTS: Most of the pDOR-SB-transfected cells demonstrated Bcl-2 positive signals, while no signal was found in the controls. The positive rate of Bcl-2 protein detected by flow cytometry in the obtained monoclonal cell strain, which was named HCC-bcl2, was 100% after the cells had been cloned 3 times continually. The killing rate, TUNEL index and the scale of sub-G1 apoptotic peak in HCC-bcl2 cells were all significantly lower than those in the control cells. CONCLUSION: Overexpression of Bcl-2 protein suppresses ethanol-induced apoptosis of the HCC cell line HCC-9204. |
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Authors:
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Lianjun Yang; Xiaohui Si; Wenliang Wang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chinese medical journal Volume: 115 ISSN: 0366-6999 ISO Abbreviation: Chin. Med. J. Publication Date: 2002 Jan |
Date Detail:
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Created Date: 2002-04-04 Completed Date: 2002-04-18 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7513795 Medline TA: Chin Med J (Engl) Country: China |
Other Details:
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Languages: eng Pagination: 8-11 Citation Subset: IM |
Affiliation:
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Department of Pathology, Fourth Military Medical University, Xi'an 710032, China. yangsi@sina.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Carcinoma, Hepatocellular / metabolism, pathology* Ethanol / pharmacology* Flow Cytometry Humans In Situ Nick-End Labeling Liver Neoplasms / metabolism, pathology* Proto-Oncogene Proteins c-bcl-2 / physiology* Transfection Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-bcl-2; 64-17-5/Ethanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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