Document Detail


Overexpression of bcl-2 protects hepatoma cell line HCC-9204 from ethanol-induced apoptosis.
MedLine Citation:
PMID:  11930666     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the effect of overexpression of bcl-2 on ethanol-induced apoptosis of primary hepatocellular carcinoma (HCC) cells. METHODS: The retrovirus expression vector pDOR-SB containing human bcl-2 cDNA was introduced into a human HCC cell line HCC-9204 by liposome-mediated transfection. pDOR-transfected and non-transfected HCC-9204 cells were used as controls. The expression of Bcl-2 protein by transfected HCC-9204 cells was detected by the immunohistochemical method. Then the cells were cloned with the limited dilution method continually until a monoclonal cell strain whose positive rate of Bcl-2 protein was 100% detected by flow cytometry was obtained. The killing rates of cells were detected by Methabenzthiazuron assay after the treatment of 6% ethanol for 6 h. The extent of apoptosis was analyzed by transferase-mediated dUTP nick end labeling (TUNEL) staining and flow cytometry. RESULTS: Most of the pDOR-SB-transfected cells demonstrated Bcl-2 positive signals, while no signal was found in the controls. The positive rate of Bcl-2 protein detected by flow cytometry in the obtained monoclonal cell strain, which was named HCC-bcl2, was 100% after the cells had been cloned 3 times continually. The killing rate, TUNEL index and the scale of sub-G1 apoptotic peak in HCC-bcl2 cells were all significantly lower than those in the control cells. CONCLUSION: Overexpression of Bcl-2 protein suppresses ethanol-induced apoptosis of the HCC cell line HCC-9204.
Authors:
Lianjun Yang; Xiaohui Si; Wenliang Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chinese medical journal     Volume:  115     ISSN:  0366-6999     ISO Abbreviation:  Chin. Med. J.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-04-04     Completed Date:  2002-04-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7513795     Medline TA:  Chin Med J (Engl)     Country:  China    
Other Details:
Languages:  eng     Pagination:  8-11     Citation Subset:  IM    
Affiliation:
Department of Pathology, Fourth Military Medical University, Xi'an 710032, China. yangsi@sina.com
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Carcinoma, Hepatocellular / metabolism,  pathology*
Ethanol / pharmacology*
Flow Cytometry
Humans
In Situ Nick-End Labeling
Liver Neoplasms / metabolism,  pathology*
Proto-Oncogene Proteins c-bcl-2 / physiology*
Transfection
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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