Document Detail

Overexpression of bcl-2 protects hepatoma cell line HCC-9204 from ethanol-induced apoptosis.
MedLine Citation:
PMID:  11930666     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate the effect of overexpression of bcl-2 on ethanol-induced apoptosis of primary hepatocellular carcinoma (HCC) cells. METHODS: The retrovirus expression vector pDOR-SB containing human bcl-2 cDNA was introduced into a human HCC cell line HCC-9204 by liposome-mediated transfection. pDOR-transfected and non-transfected HCC-9204 cells were used as controls. The expression of Bcl-2 protein by transfected HCC-9204 cells was detected by the immunohistochemical method. Then the cells were cloned with the limited dilution method continually until a monoclonal cell strain whose positive rate of Bcl-2 protein was 100% detected by flow cytometry was obtained. The killing rates of cells were detected by Methabenzthiazuron assay after the treatment of 6% ethanol for 6 h. The extent of apoptosis was analyzed by transferase-mediated dUTP nick end labeling (TUNEL) staining and flow cytometry. RESULTS: Most of the pDOR-SB-transfected cells demonstrated Bcl-2 positive signals, while no signal was found in the controls. The positive rate of Bcl-2 protein detected by flow cytometry in the obtained monoclonal cell strain, which was named HCC-bcl2, was 100% after the cells had been cloned 3 times continually. The killing rate, TUNEL index and the scale of sub-G1 apoptotic peak in HCC-bcl2 cells were all significantly lower than those in the control cells. CONCLUSION: Overexpression of Bcl-2 protein suppresses ethanol-induced apoptosis of the HCC cell line HCC-9204.
Lianjun Yang; Xiaohui Si; Wenliang Wang
Related Documents :
9667736 - Genistein modulates neuroblastoma cell proliferation and differentiation through induct...
11724306 - Interleukin-6 inhibits radiation induced apoptosis in pancreatic cancer cells.
11337016 - Labdane type diterpenes down-regulate the expression of c-myc protein, but not of bcl-2...
7845006 - Phosphorothioate bcr-abl antisense oligonucleotides induce cell death, but fail to redu...
21354126 - Asymmetric localization of germline markers vasa and nanos during early development in ...
2051076 - Division-associated and division-independent hyperthermic cell death: comparison with o...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chinese medical journal     Volume:  115     ISSN:  0366-6999     ISO Abbreviation:  Chin. Med. J.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-04-04     Completed Date:  2002-04-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7513795     Medline TA:  Chin Med J (Engl)     Country:  China    
Other Details:
Languages:  eng     Pagination:  8-11     Citation Subset:  IM    
Department of Pathology, Fourth Military Medical University, Xi'an 710032, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Apoptosis / drug effects*
Carcinoma, Hepatocellular / metabolism,  pathology*
Ethanol / pharmacology*
Flow Cytometry
In Situ Nick-End Labeling
Liver Neoplasms / metabolism,  pathology*
Proto-Oncogene Proteins c-bcl-2 / physiology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Analysis of the GM-CSF and GM-CSF/IL-3/IL-5 receptor common beta chain in a patient with pulmonary a...
Next Document:  Acute effect of tetrandrine pulmonary targeting microspheres on hypoxic pulmonary hypertension in ra...