Document Detail

Overexpression of alpha-catenin increases osteoblastic differentiation in mouse mesenchymal C3H10T1/2 cells.
MedLine Citation:
PMID:  19324011     Owner:  NLM     Status:  MEDLINE    
alpha- and beta-Catenin link cadherins to the actin-based cytoskeleton at adherens junctions and regulate cell-cell adhesion. Although roles of cadherins and canonical Wnt-/beta-catenin-signaling in osteoblastic differentiation have been extensively studied, the role of alpha-catenin is not known. Murine embryonic mesenchymal stem cells, C3H10T1/2 cells, were transduced with retrovirus encoding alpha-catenin (MSCV-alpha-catenin-HA-GFP). In the presence of Wnt-3A conditioned medium or osteogenic medium (beta-glycerol phosphate and ascorbic acid), cells overexpressing alpha-catenin showed enhanced osteoblastic differentiation as measured by alkaline phosphatase (ALP) staining and ALP activity assay compared to cells transduced with empty virus (MSCV-GFP). In addition, mRNA expression of osteocalcin and Runx2 was significantly increased compared to control. Cell aggregation assay revealed that alpha-catenin overexpression has significantly increased cell-cell aggregation. However, cellular beta-catenin levels (total, cytoplasmic-nuclear ratio) and beta-catenin-TCF/LEF transcriptional activity did not change by overexpression of alpha-catenin. Knock-down of alpha-catenin using siRNA decreased osteoblastic differentiation as measured by ALP assay. These results suggest that alpha-catenin overexpression increases osteoblastic differentiation by increasing cell-cell adhesion rather than Wnt-/beta-catenin-signaling.
Dohee Kim; Jae-Yeon Yang; Chan Soo Shin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-24
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  382     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-22     Completed Date:  2009-05-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  745-50     Citation Subset:  IM    
Department of Internal Medicine, Dankook University College of Medicine, Cheonan, Republic of Korea.
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MeSH Terms
Cell Adhesion
Cell Differentiation* / genetics
Cell Line
Mesenchymal Stem Cells / metabolism,  physiology*
Osteoblasts / cytology*,  metabolism
RNA, Small Interfering / genetics
Transcription, Genetic
alpha Catenin / biosynthesis*,  genetics
beta Catenin / metabolism
Reg. No./Substance:
0/RNA, Small Interfering; 0/alpha Catenin; 0/beta Catenin

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