Document Detail


Overexpression of acyl carrier protein-1 alters fatty acid composition of leaf tissue in Arabidopsis.
MedLine Citation:
PMID:  11553750     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acyl carrier protein (ACP) is a small (9 kD) acidic protein that is an essential cofactor in plant fatty acid biosynthesis. Most plants have several isoforms of ACP, some of which are expressed constitutively and others that appear to be more tissue specific. Although the critical role of ACP in fatty acid biosynthesis has been established, the role of the diverse number of isoforms has yet to be elucidated. We have generated transgenic Arabidopsis plants that express high levels of ACP-1, a seed-predominant ACP isoform, in leaf tissue under control of the cauliflower mosaic virus 35S promoter. Western and northern analysis of these plants demonstrate 3- to 8-fold increased expression of this isoform in leaf tissue, but no significant changes in seed. Analysis of the fatty acid composition of leaf tissue revealed that overexpression of ACP-1 in leaf tissue alters fatty acid composition. Significant decreases in levels of 16:3 were noted along with increases in 18:3. These findings represent the first in vivo report that overexpression of an ACP isoform results in changes in fatty acid composition in plants.
Authors:
J K Branen; T J Chiou; N J Engeseth
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant physiology     Volume:  127     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-12     Completed Date:  2001-12-04     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  222-9     Citation Subset:  IM    
Affiliation:
University of Illinois, Department of Food Science and Human Nutrition, 259 ERML, 1201 West Gregory, Urbana, Illinois 61801, USA.
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MeSH Terms
Descriptor/Qualifier:
Acyl Carrier Protein / genetics,  metabolism*
Arabidopsis / genetics,  metabolism*
Blotting, Western
Caulimovirus / genetics
Fatty Acids / biosynthesis*,  chemistry
Genetic Vectors
Lipid Metabolism*
Lipids / chemistry
Organ Specificity
Plant Leaves / genetics,  metabolism
Plants, Genetically Modified
Protein Isoforms / genetics,  metabolism
Seeds / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Acyl Carrier Protein; 0/Fatty Acids; 0/Lipids; 0/Protein Isoforms
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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