| Overexpression and activation of the RON receptor tyrosine kinase in a panel of human colorectal carcinoma cell lines. | |
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MedLine Citation:
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PMID: 11082293 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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RON is a receptor tyrosine kinase belonging to the MET proto-oncogene family. The purposes of this study are to determine the expression and activation of RON in a panel of human colon carcinoma cell lines. Western blotting showed that RON is barely detectable in normal and SV-40-transformed colon epithelial cells, but highly expressed and constitutively activated in several colon carcinoma cell lines including Colo201, HT-29, HCT116, and SW837. Moreover, a novel RON variant with a molecular mass of 160 kDa (RONDelta160) was identified from HT-29 cells. The cDNA encoding RONDelta160 has an in-frame deletion of 109 amino acids in the extracellular domain of the RON beta chain, which is caused by splicing out of two exons in the RON mRNA. No mutations were found in the kinase domain of the RON gene in five carcinoma cell lines screened. By expressing RON in colon epithelial cells, we found that RON activation increases cell motile-invasive activities and protects cells against apoptotic death. These data suggest that RON expression and activation are deregulated in colon carcinoma cell lines. By abnormal activation of RON, this receptor and its variant may regulate motile-invasive phenotypes of certain colon carcinoma cells in vivo. |
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Authors:
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Y Q Chen; Y Q Zhou; D Angeloni; A L Kurtz; X Z Qiang; M H Wang |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental cell research Volume: 261 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-12-22 Completed Date: 2000-12-22 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 229-38 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Academic Press. |
Affiliation:
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Department of Medicine, University of Colorado School of Medicine and Denver Health Medical Center, Denver, Colorado 80204, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cell Line Colon Colonic Neoplasms / enzymology* Colorectal Neoplasms / enzymology* Dogs Enzyme Activation Humans Intestinal Mucosa / enzymology Molecular Sequence Data Receptor Protein-Tyrosine Kinases / chemistry, genetics*, metabolism* Receptors, Cell Surface / chemistry, genetics*, metabolism* Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Transfection Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI43516/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Cell Surface; 0/Recombinant Proteins; EC 2.7.1.-/RON protein; EC 2.7.10.1/Receptor Protein-Tyrosine Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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