| Overexpression of a Zn2+-sensitive soluble exopolyphosphatase from Trypanosoma cruzi depletes polyphosphate and affects osmoregulation. | |
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MedLine Citation:
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PMID: 17827150 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report the cloning, expression, purification, and characterization of the Trypanosoma cruzi exopolyphosphatase (TcPPX). The product of this gene (TcPPX), has 383 amino acids and a molecular mass of 43.1 kDa. TcPPX differs from most exopolyphosphatases in its preference for short-chain polyphosphate (poly P). Heterologous expression of TcPPX in Escherichia coli produced a functional enzyme that had a neutral optimum pH and was dramatically inhibited by low concentrations of Zn2+, high concentrations of basic amino acids (lysine and arginine), and heparin. TcPPX is a processive enzyme and does not hydrolyze ATP, pyrophosphate, or p-nitrophenyl phosphate, although it hydrolyzes guanosine 5'-tetraphosphate very efficiently. Overexpression of TcPPX resulted in a dramatic decrease in total short-chain poly P and partial decrease in long-chain poly P. This was accompanied by a delayed regulatory volume decrease after hyposmotic stress. These results support the role of poly P in T. cruzi osmoregulation. |
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Authors:
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Jianmin Fang; Felix A Ruiz; Melissa Docampo; Shuhong Luo; Juliany C F Rodrigues; Lucimar S Motta; Peter Rohloff; Roberto Docampo |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-09-07 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 282 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-10-29 Completed Date: 2007-12-13 Revised Date: 2011-04-21 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 32501-10 Citation Subset: IM |
Affiliation:
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Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Paul D. Coverdell Biomedical and Health Sciences Center, Athens, Georgia 30602, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF545106; AY178275 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acid Anhydride Hydrolases
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chemistry,
genetics,
metabolism* Amino Acid Sequence Animals Cloning, Molecular Molecular Sequence Data Polyphosphates / metabolism* Protein Structure, Tertiary Protozoan Proteins / chemistry, genetics, metabolism* Recombinant Proteins / chemistry, genetics, metabolism Sequence Homology Trypanosoma cruzi / enzymology* Water-Electrolyte Balance* Zinc / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AI68647/AI/NIAID NIH HHS; R01 AI068647-02/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Polyphosphates; 0/Protozoan Proteins; 0/Recombinant Proteins; 7440-66-6/Zinc; EC 3.6.-/Acid Anhydride Hydrolases; EC 3.6.1.11/exopolyphosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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