| Overexpression of the β Subunit of Human Chorionic Gonadotropin Promotes the Transformation of Human Ovarian Epithelial Cells and Ovarian Tumorigenesis. | |
| | |
MedLine Citation:
|
PMID: 21763678 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Ovarian carcinoma is the most lethal gynecologic malignancy, but underlying molecular events remain elusive. Expression of human chorionic gonadotropin β subunit (β-hCG) is clinically significant for both trophoblastic and nontrophoblastic cancers; however, whether β-hCG facilitates ovarian epithelial cell tumorigenic potential has been uncharacterized. Immortalized nontumorigenic ovarian epithelial T29 and T80 cells stably overexpressing β-hCG were examined for alterations in cell cycle and apoptotic status by flow cytometry, expression of proteins regulating cell cycle and apoptosis by Western blot, proliferation status by MTT assay, anchorage-independent colony formation, and mouse tumor formation. β-hCG IHC reactivity was evaluated on mouse xenografts and on human normal ovarian, fallopian tube, endometrium, and ovarian carcinoma tissues. T29 and T80 cells overexpressing β-hCG demonstrated significantly increased proliferation, anchorage-independent colony formation, prosurvival Bcl-X(L) protein expression, G2-phase progression, elevated cyclins E/D1 and Cdk 2/4/6, and decreased apoptosis. Collectively, these transformational parameters facilitated increased xenograft tumorigenesis (P < 0.05). β-hCG was elevated in malignant ovarian tumors, compared with normal epithelial expression in ovaries, fallopian tube, and endometrium (P < 0.001). Our data indicate that elevated β-hCG transforms ovarian surface epithelial cells, facilitating proliferation, cell cycle progression, and attenuated apoptosis to promote tumorigenesis. Our results further decipher the functional role and molecular mechanism of β-hCG in ovarian carcinoma. β-hCG may contribute to ovarian cancer etiology, which introduces a new therapeutic intervention target for ovarian cancer. |
| | |
Authors:
|
Xiaoqing Guo; Guangzhi Liu; Isaiah G Schauer; Gong Yang; Imelda Mercado-Uribe; Fan Yang; Shiwu Zhang; Yuanli He; Jinsong Liu |
Related Documents
:
|
22558358 - Exosomes released from mycoplasma infected tumor cells activate inhibitory b cells. 4046028 - Constraints on flagellar rotation. 17536538 - Cytologic characteristics of subependymal giant cell astrocytoma in squash smears: morp... 3928848 - Growth behavior and lineage of isolated and cultured cells derived from giant cell gran... 20498638 - Evidence for mesenchymal-like sub-populations within squamous cell carcinomas possessin... 11351298 - Overexpression of lysosomal-type sialidase leads to suppression of metastasis associate... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-7-14 |
Journal Detail:
|
Title: The American journal of pathology Volume: - ISSN: 1525-2191 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
|
Created Date: 2011-7-18 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, China; Department of Pathology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas; Department of Obstetrics and Gynecology, Shihezi University School of Medicine, Shihezi, Xinjiang, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Neutrophil Elastase Is Produced by Pulmonary Artery Smooth Muscle Cells and Is Linked to Neointimal ...
Next Document: Orally Administered Prion Protein Is Incorporated by M Cells and Spreads into Lymphoid Tissues with ...