Document Detail


Overexpression of SPINDLIN1 induces cellular senescence, multinucleation and apoptosis.
MedLine Citation:
PMID:  18201843     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human or mouse Spindlin1 is expressed in various tissues and cells, but its biological functions are poorly understood. In this study, we show that human SPINDLIN1 is localized to interphase nucleus and mitotic chromosomes, and its expression in HeLa cells is not regulated in a cell cycle-dependent manner. When SPINDLIN1 is stably overexpressed in HeLa cells, it results in multinucleation of cells, and these multinucleated cells exhibits characteristic features of senescence and apoptosis shown by growth and morphological alterations, beta-galactosidase activity, and Annexin V/7-Aminoactinomycin D staining. Mouse Spindlin1 is highly homologous with human Spindlin1, when overexpressed in NIH3T3 cells, it also induces multinucleation, senescence and apoptosis in murine cells. Our results demonstrate that SPINDLIN1 is an important gene for mammalian mitotic chromosome functions, and disrupted regulation results in abnormal cell division, a mechanism that may be involved in tumorigenesis.
Authors:
Hongfeng Yuan; Peng Zhang; Lipeng Qin; Lin Chen; Shuangshuang Shi; Yang Lu; Fang Yan; Cixian Bai; Xue Nan; Daqing Liu; Yanhua Li; Wen Yue; Xuetao Pei
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-14
Journal Detail:
Title:  Gene     Volume:  410     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-22     Completed Date:  2008-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  67-74     Citation Subset:  IM    
Affiliation:
Laboratory of Stem Cell and Regeneration Medicine, Beijing Institute of Transfusion Medicine, 27 Taiping Road, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Apoptosis / genetics*
Cell Aging / genetics*
Cell Cycle Proteins / chemistry,  genetics*
Hela Cells
Humans
Mice
Microtubule-Associated Proteins / chemistry,  genetics*
Molecular Sequence Data
NIH 3T3 Cells
Phosphoproteins / chemistry,  genetics*
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Microtubule-Associated Proteins; 0/Phosphoproteins; 0/spindlin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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