Document Detail


Overexpression of SERCA1a in the mdx diaphragm reduces susceptibility to contraction-induced damage.
MedLine Citation:
PMID:  20540606     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although the precise pathophysiological mechanism of muscle damage in dystrophin-deficient muscle remains disputed, calcium appears to be a critical mediator of the dystrophic process. Duchenne muscular dystrophy patients and mouse models of dystrophin deficiency exhibit extensive abnormalities of calcium homeostasis, which we hypothesized would be mitigated by increased expression of the sarcoplasmic reticulum calcium pump. Neonatal adeno-associated virus gene transfer of sarcoplasmic reticulum ATPase 1a to the mdx diaphragm decreased centrally located nuclei and resulted in reduced susceptibility to eccentric contraction-induced damage at 6 months of age. As the diaphragm is the mouse muscle most representative of human disease, these results provide impetus for further investigation of therapeutic strategies aimed at enhanced cytosolic calcium removal.
Authors:
Kevin J Morine; Meg M Sleeper; Elisabeth R Barton; H Lee Sweeney
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Human gene therapy     Volume:  21     ISSN:  1557-7422     ISO Abbreviation:  Hum. Gene Ther.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-14     Completed Date:  2011-06-29     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9008950     Medline TA:  Hum Gene Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1735-9     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA. kmorine@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Dependovirus / genetics
Diaphragm / metabolism*,  physiopathology
Gene Transfer Techniques
Genetic Therapy
Genetic Vectors
Humans
Mice
Mice, Inbred C57BL
Mice, Inbred mdx
Muscle Contraction*
Muscle Strength
Protein Isoforms / biosynthesis
Recombinant Proteins / biosynthesis*
Sarcoplasmic Reticulum Calcium-Transporting ATPases / biosynthesis*
Transgenes
Grant Support
ID/Acronym/Agency:
U54-AR052646/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Protein Isoforms; 0/Recombinant Proteins; EC 3.6.3.8/Sarcoplasmic Reticulum Calcium-Transporting ATPases
Comments/Corrections

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