Document Detail


Overexpression, purification, and biochemical characterization of the esterase Est0796 from Lactobacillus plantarum WCFS1.
MedLine Citation:
PMID:  23054632     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lactobacillus plantarum is a lactic acid bacterium that is encountered in an extensive range of foods such as fermented dairy products, meat, vegetables, and bakery products. Given the little molecular information available on the lipolytic activity of L. plantarum, the aim of this study was to clone, purify, and biochemically characterize the esterase coded by gene lp_0796 (Est0796). The esterase was cloned in pET28a and purified in two steps, using solid ammonium sulfate and His tag affinity chromatography. The molecular mass of the purified Est0796 was 28.7 kDa (by SDS-PAGE) and 26.6 kDa (by gel filtration chromatography), pointing to a monomeric structure. Est0796 showed maximum activity at pH 8.0 and 35 °C and toward shorter acyl chain lengths (C2-C4). The activity was resistant to organic solvents and cations, suggesting that this esterase may play a role in the fermentation of food products.
Authors:
Inmaculada Navarro-González; Navarro-González Inmaculada; Sánchez-Ferrer Alvaro; García-Carmona Francisco
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular biotechnology     Volume:  54     ISSN:  1559-0305     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-04-26     Completed Date:  2013-12-30     Revised Date:  2014-08-03    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  651-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Bacterial Proteins / genetics*,  metabolism
Chromatography, Gel / methods
Cloning, Molecular / methods
Dairy Products / microbiology
Esterases / genetics*,  metabolism
Fermentation
Food Microbiology
Hydrogen-Ion Concentration
Lactobacillus plantarum / enzymology,  genetics*,  metabolism
Molecular Sequence Data
Molecular Weight
Recombinant Proteins
Sequence Alignment
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Recombinant Proteins; EC 3.1.-/Esterases
Comments/Corrections
Erratum In:
Mol Biotechnol. 2013 Oct;55(2):168
Note: Inmaculada, Navarro-González [corrected to Navarro-González, Inmaculada]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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