Document Detail


Overexpression of Plk1 promotes malignant progress in human esophageal squamous cell carcinoma.
MedLine Citation:
PMID:  19572149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Plk1, belonging to a family of serine/threonine kinases, is involved in spindle formation and chromosome segregation during mitosis and therefore, in the regulation of cell cycle. Plk1 was found to be overexpressed in various human tumors. In the present work, we investigated the expression of human esophageal squamous cell carcinoma (ESCC) to determine whether Plk1 has a role in malignant progress.
METHODS: Immunohistochemistry and Western blotting were performed to define the expressions of Plk1 in ESCC tissues and normal adjacent tissues. Transfection of cells with small interference RNA, growth suppression assay, and Transwell assay were used to determine the potential role of Plk1 in ESCC malignant progress.
RESULTS: Plk1 was overexpressed in 69.6% of the ESCC tissues. In addition, the extent of Plk1 expression was closely correlated with differentiation grades and invasiveness in ESCC. We also found that the downregulation of endogenous Plk1 in human ESSC cell lines Eca-109 and EC9706 significantly decreased cells proliferation and migrating ability.
CONCLUSIONS: Our results show that Plk1 expression is elevated in ESCC tissues and is associated with differentiation grades and invasiveness in ESCC, indicating that overexpression of Plk1 may play an important role in carcinogenesis and malignant progress of ESCC.
Authors:
Chunling Zhao; Lei Gong; Wentong Li; Limei Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cancer research and clinical oncology     Volume:  136     ISSN:  1432-1335     ISO Abbreviation:  J. Cancer Res. Clin. Oncol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2011-04-06     Completed Date:  2011-05-05     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  7902060     Medline TA:  J Cancer Res Clin Oncol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  9-16     Citation Subset:  IM    
Affiliation:
Basic Medicine Department, Weifang Medical University, 261053 Weifang, Shandong, People's Republic of China. linglinghappy2003@yahoo.com.cn
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Carcinoma, Squamous Cell / genetics,  metabolism*,  pathology
Cell Cycle Proteins / genetics,  metabolism*
Cell Line, Tumor
Cell Movement
Cell Proliferation
Disease Progression
Esophageal Neoplasms / genetics,  metabolism*,  pathology
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Protein-Serine-Threonine Kinases / genetics,  metabolism*
Proto-Oncogene Proteins / genetics,  metabolism*
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Proto-Oncogene Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/polo-like kinase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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