Document Detail


Overexpression of the Parkinson disease protein DJ-1 and its regulator PTEN in gestational trophoblastic disease.
MedLine Citation:
PMID:  20736773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DJ-1 is found to be important in human neurodegenerative diseases and cancers by regulating oxidative damage and apoptosis. DJ-1 is also a regulator of PTEN, a frequently mutated tumor suppressor gene in cancers. In this study, we investigated the expression of DJ-1 and PTEN in normal placentas and gestational trophoblastic disease in relation to apoptotic indices and p53 status. A total of 95 trophoblastic samples were retrieved for immunohistochemical study whereas 79 trophoblastic samples, 3 normal trophoblastic and 2 choriocarcinoma cell lines were collected for quantitative real time reverse transcription polymerase chain reaction detection. There was a significant correlation between DJ-1 and PTEN immunostaining indices in the trophoblastic samples (P=0.013). Significantly higher DJ-1 and PTEN immunoreactivity indices were found in the complete mole (P<0.01) and choricarcinoma (P<0.01) compared with the first trimester placenta. Quantitative real time reverse transcription polymerase chain reaction also detected significantly higher messenger ribonucleic acid expressions of DJ-1 and PTEN in hydatidiform moles (P<0.05) and choriocarcinomas (P<0.05) compared with the first trimester placentas. A significant negative correlation was found between DJ-1 and the apoptosis resistant gene Bcl-2 (P=0.031), whereas a positive correlation was shown between PTEN and wild-type p53 (P=0.019). Significant correlations between PTEN and embryonic stem cell transcription factors, Stat3 and Nanog, were also displayed (P=0.001, 0.015). Our findings showed, for the first time, overexpression of DJ-1 at both transcriptional and protein levels in gestational trophoblastic disease. Overexpressed DJ-1 may play a role in regulating apoptotic activities of trophoblasts in relation to PTEN and p53.
Authors:
Hui-Juan Zhang; Michelle Kwan-Yee Siu; Li-Li Jiang; Victor Chun-Yin Mak; Hextan Yuen-Sheung Ngan; Annie Nga-Yin Cheung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists     Volume:  29     ISSN:  1538-7151     ISO Abbreviation:  Int. J. Gynecol. Pathol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-25     Completed Date:  2010-12-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8214845     Medline TA:  Int J Gynecol Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  468-75     Citation Subset:  IM    
Affiliation:
Department of Pathology, Queen Mary Hospital, the University of Hong Kong, Pokfulam Road, Hong Kong, China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / physiology
Female
Gene Expression Regulation, Neoplastic*
Gestational Trophoblastic Neoplasms / genetics*,  metabolism,  pathology
Humans
Immunohistochemistry
Intracellular Signaling Peptides and Proteins / genetics
Oncogene Proteins / biosynthesis*,  genetics
PTEN Phosphohydrolase / biosynthesis*,  genetics
Placenta / metabolism,  pathology
Pregnancy
Reverse Transcriptase Polymerase Chain Reaction
Uterine Neoplasms / genetics*,  metabolism,  pathology
Chemical
Reg. No./Substance:
0/Intracellular Signaling Peptides and Proteins; 0/Oncogene Proteins; 0/PARK7 protein, human; EC 3.1.3.48/PTEN protein, human; EC 3.1.3.67/PTEN Phosphohydrolase

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