Document Detail


Overexpression of Hp95 induces G1 phase arrest in confluent HeLa cells.
MedLine Citation:
PMID:  11683497     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Xp95, a protein recently identified in Xenopus laevis, is potentially involved in progesterone-induced Xenopus oocyte maturation. In this study, we cloned a human homologue of Xp95, designated Hp95, and examined the effect of its overexpression on the growth properties of human malignant HeLa cells which have lost the contact inhibition of cell proliferation. We observed that although HeLa cells did not undergo G1 phase arrest at any stage after confluence, they were able to downregulate their G1 phase CDK activities in response to confluence. When Hp95 was overexpressed in HeLa cells by transfection with a constitutive or an inducible expression vector containing a full-length Hp95 transgene, HeLa cells became able to undergo G1 phase arrest and form a monolayer culture after confluence. However, the G1 phase CDK activities in these Hp95 overexpressing cells were not inhibited further as compared to control cells after confluence. These results indicate that the defects in HeLa cells that cause the loss of contact inhibition of cell proliferation are in components downstream of the G1 phase CDKs and that overexpression of Hp95 counteracts some of these defects.
Authors:
Y Wu; S Pan; S Che; G He; M Nelman-Gonzalez; M M Weil; J Kuang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  67     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-10-30     Completed Date:  2002-01-17     Revised Date:  2012-06-25    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  139-53     Citation Subset:  IM    
Affiliation:
Department of Experimental Therapeutics, The University of Texas, M. D. Anderson Cancer Center, Houston 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Blotting, Western
Calcium-Binding Proteins
Carrier Proteins
Cell Cycle Proteins / chemistry,  genetics*,  metabolism*
Cell Division / genetics
Cloning, Molecular
Cyclin-Dependent Kinase 4
Cyclin-Dependent Kinases / metabolism
Endosomal Sorting Complexes Required for Transport
Flow Cytometry
G1 Phase*
Gene Expression
HeLa Cells
Humans
Molecular Sequence Data
Phosphoproteins / chemistry,  genetics*,  metabolism*
Proto-Oncogene Proteins*
RNA, Messenger / genetics,  metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Time Factors
Transgenes / genetics
Grant Support
ID/Acronym/Agency:
R-29 GM48457-02/GM/NIGMS NIH HHS; R01 CA77332/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/Carrier Proteins; 0/Cell Cycle Proteins; 0/Endosomal Sorting Complexes Required for Transport; 0/PDCD6IP protein, human; 0/Phosphoproteins; 0/Proto-Oncogene Proteins; 0/RNA, Messenger; EC 2.7.11.22/CDK4 protein, human; EC 2.7.11.22/Cyclin-Dependent Kinase 4; EC 2.7.11.22/Cyclin-Dependent Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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