Document Detail


Overexpression of GLUTAMINE DUMPER1 leads to hypersecretion of glutamine from Hydathodes of Arabidopsis leaves.
MedLine Citation:
PMID:  15208395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Secretion is a fundamental process providing plants with the means for disposal of solutes, improvement of nutrient acquisition, and attraction of other organisms. Specific secretory organs, such as nectaries, hydathodes, and trichomes, use a combination of secretory and retrieval mechanisms, which are poorly understood at present. To study the mechanisms involved, an Arabidopsis thaliana activation tagged mutant, glutamine dumper1 (gdu1), was identified that accumulates salt crystals at the hydathodes. Chemical analysis demonstrated that, in contrast with the amino acid mixture normally present in guttation droplets, the crystals mainly contain Gln. GDU1 was cloned and found to encode a novel 17-kD protein containing a single putative transmembrane span. GDU1 is expressed in the vascular tissues and in hydathodes. Gln content is specifically increased in xylem sap and leaf apoplasm, whereas the content of several amino acids is increased in leaves and phloem sap. Selective secretion of Gln by the leaves may be explained by an enhanced release of this amino acid from cells. GDU1 study may help to shed light on the secretory mechanisms for amino acids in plants.
Authors:
Guillaume Pilot; Harald Stransky; Dean F Bushey; Réjane Pratelli; Uwe Ludewig; Vincent P M Wingate; Wolf B Frommer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-06-18
Journal Detail:
Title:  The Plant cell     Volume:  16     ISSN:  1040-4651     ISO Abbreviation:  Plant Cell     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-05     Completed Date:  2004-11-01     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1827-40     Citation Subset:  IM    
Affiliation:
Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universität Tübingen, D-72076 Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / metabolism*
Arabidopsis / genetics*,  metabolism
Arabidopsis Proteins / genetics*
DNA, Bacterial / genetics
Gene Expression Regulation, Plant*
Membrane Proteins / physiology
Molecular Sequence Data
Multigene Family
Mutation
Phenotype
Plant Leaves / metabolism*
Plant Roots / metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Arabidopsis Proteins; 0/DNA, Bacterial; 0/GDU1 protein, Arabidopsis; 0/Membrane Proteins; 0/T-DNA
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