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Overexpression of CD9 in Human Breast Cancer Cells Promotes the Development of Bone Metastases.
MedLine Citation:
PMID:  23225418     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND: Bone is a preferred target for circulating metastatic breast cancer cells. We found that the CD9 protein was up-regulated in the B02 osteotropic cell line, derived from the aggressive parental MDA-MB-231 breast cancer cell line. Here, we investigated the putative relationship between CD9 expression and the osteotropic phenotype.
MATERIALS AND METHODS: Overexpression of CD9 was analyzed by immunoblotting in different cell lines. Immunohistochemistry was used to assess CD9 expression in primary tumors and metastatic lesions. In vivo experiments were conducted in mice using a monoclonal antibody against CD9.
RESULTS: CD9 overexpression was confirmed in osteotropic cells. CD9 was significantly overexpressed in bone metastases versus primary tumors and visceral metastatic lesions. Finally, in vivo experiments showed that an antibody against CD9 delays homing of B02 cells in bone marrow, slowing down bone destruction.
CONCLUSION: Our study reveals a potential implication of CD9 in the formation of bony metastases from breast cancer cells.
Authors:
Philippe Kischel; Akeila Bellahcene; Blandine Deux; Virginie Lamour; Rowan Dobson; Edwin DE Pauw; Philippe Clezardin; Vincent Castronovo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  32     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  5211-20     Citation Subset:  IM    
Affiliation:
Laboratoire de Physiologie Cellulaire et Moléculaire, Université d'Amiens, UFR des Sciences, 33 Rue Saint-Leu, 80039 Amiens Cedex 1, France. Philippe.Kischel@u-picardie.fr.
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