Document Detail


Overexpression of Bax inhibitor-1 (BI-1) induces cell transformation in NIH3T3 cells.
MedLine Citation:
PMID:  20597862     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BI-1 (Bax inhibitor-1), an apoptosis-inhibiting gene belonging to the Bcl-2 protein family, plays an important role in mitochondrial apoptosis pathway to suppress Bax-induced apoptosis. To investigate the potential role of BI-1 in promoting cell growth and tumorigenesis, in the present study we overexpressed the BI-1 gene in NIH3T3 cells using the lentivirus-mediated gene expression system. Our in vitro studies showed that NIH3T3 cells overexpressing BI-1 displayed a significantly higher growth rate and formed more and larger colonies than the control cells. In addition, our in vivo studies indicated that the lenti-BI-1-infected cells formed obvious tumours, while no tumours were formed by the control cells after subcutaneously injected into nude mice. These results strongly suggested that the BI-1 gene might play a crucial role in neoplastic genesis and development.
Authors:
Li Xiang-yong; Chen Yang-chao; Zhou Ke-yuan; Zhang Mei-hong; Luo Hai-qing; Kung Hsiang-fu; Zhou Xin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  34     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-13     Completed Date:  2011-02-07     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  1099-104     Citation Subset:  IM    
Affiliation:
Center of Clinical Gene Diagnosis, Zhongnan Hospital of Wuhan University, Hubei, China. xyli75@126.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Apoptosis Regulatory Proteins / genetics,  metabolism*
Cell Transformation, Neoplastic* / genetics
Cells, Cultured
Gene Expression Regulation
Humans
Membrane Proteins / genetics,  metabolism*
Mice
Mice, Nude
NIH 3T3 Cells
Transfection
Chemical
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/Membrane Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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