Document Detail


Overexpression of ADP-Ribosylation factor 1 in Human Gastric Carcinoma and its Clinicopathological Significance.
MedLine Citation:
PMID:  22348287     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Gastric cancer is the sixth leading cause of cancer-related death in Taiwan, and the identification of related factors is essential to increase patient survival. ADP ribosylation factor 1 (ARF1) was initially identified using two-dimensional electrophoresis (2-DE) combined with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF). ARF1 belongs to the Ras superfamily or GTP-binding protein family and has been shown to enhance cell proliferation. In the current study, we evaluated the potential of ARF1 as a biomarker for gastric cancer detection. ARF1 mRNA was upregulated in tumor tissues (compared with adjacent non-tumor tissues, n=55) in about 67.2% of gastric cancer patients. Expression of ARF1 protein was additionally observed using western blot and Immunohistochemistry (IHC) analyses. The clinicopathological correlations of ARF1 were further evaluated. Elevated ARF1 expression was strongly correlated with lymph node metastasis (p = 0.008), serosal invasion (p =0.046), lymphatic invasion (p = 0.035) and pathological staging (p = 0.010). Moreover, the 5-year survival rate for the lower ARF1 expression group (n = 50; IHC score <90) was higher than that of the higher expression group (n = 60; IHC score ≥90) (log rank p = 0.0228). To establish the specific function of ARF1 in human gastric cancer, isogenic ARF1-overexpressing cell lines were prepared. Our results demonstrate that ARF1-overexpressing clones display enhanced cell proliferation, migration and invasion. Furthermore, ARF1-overexpression may contribute to poor prognosis of patients. These findings collectively support the utility of ARF1 as a novel prognostic marker for gastric cancer and its role in cell invasion. © 2012 Japanese Cancer Association.
Authors:
Ming-Ming Tsai; Paul Y Lin; Wan-Li Cheng; Chung-Ying Tsai; Hsiang-Cheng Chi; Cheng-Yi Chen; Yi-Hsin Tseng; Yi-Fen Cheng; Chi-De Chen; Ying Liang; Chia-Jung Liao; Sheng-Ming Wu; Yang-Hsiang Lin; I-Hsiao Chung; Chia-Siu Wang; Kwang-Huei Lin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-20
Journal Detail:
Title:  Cancer science     Volume:  -     ISSN:  1349-7006     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101168776     Medline TA:  Cancer Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Japanese Cancer Association.
Affiliation:
Graduate Institute of Biomedical Sciences, College of Medline, Chang-Gung University, Taoyuan, Taiwan, 333; Department of Nursing, Chang-Gung University of Science and Technology, Taoyuan, Taiwan, 333.
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