| Over-synthesis and instability of sigma protein in a merodiploid strain of Escherichia coli. | |
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MedLine Citation:
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PMID: 345086 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have used two different methods to study the rates of RNA polymerase subunit synthesis in haploid Escherichia coli K12, and a KLF10 rPOB, C+ merodiploid derivative, when grown in glucose-minimal medium at 37 degrees C. Our results indicate that the haploid strain produces beta, beta', alpha and sigma in the molar ratios 1.01:0.99: less than or equal to 2.90:0.26; and that all these subunits are reasonably stable during subsequent growth. The merodiploid produces alpha at the same rate as the haploid, beta and beta' at a 42% higher rate, and sigma at twice the rate. Some 40% of the newly synthesised beta and beta' is degraded within one hour; the residuum is as stable as in the haploid. Alpha is stable throughout. By contrast, sigma is subject to a marked and continuous turnover in the merodiploid. These results are discussed in terms of gene dosage and regulatory effects. |
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Authors:
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R S Hayward; S Fyfe |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular & general genetics : MGG Volume: 159 ISSN: 0026-8925 ISO Abbreviation: Mol. Gen. Genet. Publication Date: 1978 Feb |
Date Detail:
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Created Date: 1978-05-20 Completed Date: 1978-05-20 Revised Date: 2002-11-01 |
Medline Journal Info:
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Nlm Unique ID: 0125036 Medline TA: Mol Gen Genet Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 89-99 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromosomes, Bacterial DNA-Directed RNA Polymerases / biosynthesis*, genetics Electrophoresis Enzyme Precursors / biosynthesis*, genetics Escherichia coli / enzymology, genetics* Isoelectric Focusing Ploidies |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Precursors; EC 2.7.7.6/DNA-Directed RNA Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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