| Over-expression and siRNA of a novel environmental lipopolysaccharide-responding gene on the cell cycle of the human hepatoma-derived cell line HepG2. | |
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MedLine Citation:
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PMID: 18068885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipopolysaccharide (LPS) is the toxic determinant for Gram-negative bacterium infection. The individual response to LPS was related to its gene background. It is necessary to identify new molecules and signaling transduction pathways about LPS. The present study was undertaken to evaluate the effects of a novel environmental lipopolysaccharide-responding (Elrg) gene on the regulation of proliferation and cell cycle of the hepatoma-derived cell line, HepG2. By means of RT-PCR, the new molecule of Elrg was generated from a human dental pulp cell cDNA library. Expression level of Elrg in HepG2 cells was remarkably upgraded by the irritation of LPS. Localization of Elrg in HepG2 cells was positioned mainly in cytoplasm. HepG2 cells were markedly arrested in the G1 phase by over-expressing Elrg. The percentage of HepG2 cells in G1 phase partly decreased after Elrg-siRNA. In conclusion, Elrg is probably correlative with LPS responding. Elrg is probably a new protein in cytoplasm which plays an important role in regulating cell cycle. The results will deepen our understanding about the potential effects of Elrg on the human hepatoma-derived cell line HepG2. |
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Authors:
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Kejun Du; Yubo Chai; Lichao Hou; Wenhui Chang; Suming Chen; Wenjing Luo; Tongjian Cai; Xiaonan Zhang; Nanchun Chen; Yaoming Chen; Jingyuan Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-04 |
Journal Detail:
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Title: Toxicology Volume: 243 ISSN: 0300-483X ISO Abbreviation: Toxicology Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2007-12-21 Completed Date: 2008-04-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0361055 Medline TA: Toxicology Country: Ireland |
Other Details:
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Languages: eng Pagination: 303-10 Citation Subset: IM |
Affiliation:
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Department of Occupational and Environmental Health, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Carcinoma, Hepatocellular / genetics, metabolism, pathology Cell Cycle / drug effects* Cell Cycle Proteins / genetics*, metabolism Cell Line, Tumor Cells, Cultured Dental Pulp / cytology, drug effects, metabolism Enzyme-Linked Immunosorbent Assay Flow Cytometry G1 Phase / drug effects Gene Expression / drug effects* Humans Immunochemistry Lipopolysaccharides / pharmacology* Liver Neoplasms / genetics, metabolism, pathology RNA, Small Interfering / genetics* Rabbits Reverse Transcriptase Polymerase Chain Reaction / methods, trends Transfection |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Lipopolysaccharides; 0/RNA, Small Interfering |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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