| Over-expression of heat shock protein 27 attenuates doxorubicin-induced cardiac dysfunction in mice. | |
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MedLine Citation:
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PMID: 17481944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Oxidative stress and myocyte apoptosis are thought to play an important role in the pathogenesis, progression and prognosis of heart failure (HF). Heat shock protein 27 (Hsp27) has been found to confer resistance to oxidative stress in cultured cells; however, the role of Hsp27 in in-vivo hearts remains to be determined. AIM: To investigate the effects of Hsp27 over-expression on doxorubicin-induced HF. METHODS AND RESULTS: Transgenic mice (TG) with cardiac specific over-expression of Hsp27 and their wild type littermates (WT) were challenged with doxorubicin (25 mg/kg, IP) to induce HF. At day 5, TG mice had significantly improved cardiac function and viability and decreased loss of heart weight following doxorubicin exposure compared with WT. In another parallel experiment, doxorubicin-induced increased levels of reactive oxygen species, protein carbonylation, apoptosis and morphologic changes were detected in the mitochondria in WT hearts, whereas these effects were markedly attenuated in TG hearts. In addition, upregulation of heat shock protein 70 and heme oxygenase-1 was present in the TG hearts after doxorubicin stimulation in comparison to WT hearts. CONCLUSION: These findings indicate that Hsp27 may play a key role in resistance to doxorubicin-induced cardiac dysfunction. |
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Authors:
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Li Liu; Xiaojin Zhang; Bo Qian; Xiaoyan Min; Xiang Gao; Chuanfu Li; Yunlin Cheng; Jun Huang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-04 |
Journal Detail:
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Title: European journal of heart failure Volume: 9 ISSN: 1388-9842 ISO Abbreviation: Eur. J. Heart Fail. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-08-13 Completed Date: 2007-11-27 Revised Date: 2011-06-08 |
Medline Journal Info:
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Nlm Unique ID: 100887595 Medline TA: Eur J Heart Fail Country: Netherlands |
Other Details:
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Languages: eng Pagination: 762-9 Citation Subset: IM |
Affiliation:
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Department of Geriatrics, First Affiliated Hospital with Nanjing Medical University, Nanjing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibiotics, Antineoplastic / adverse effects Apoptosis / physiology Blotting, Western Disease Models, Animal Doxorubicin / adverse effects HSP27 Heat-Shock Proteins Heart Failure / chemically induced, metabolism, physiopathology* Heat-Shock Proteins / metabolism* Immunohistochemistry In Situ Nick-End Labeling Mice Mice, Transgenic Mitochondria, Heart / metabolism* Myocytes, Cardiac / ultrastructure Organ Size / drug effects Oxidative Stress / drug effects Reactive Oxygen Species / analysis |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/HSP27 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/Hspb2 protein, mouse; 0/Reactive Oxygen Species; 23214-92-8/Doxorubicin |
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