| Over-expression of hTERT in CHO K1 results in decreased apoptosis and reduced serum dependency. | |
| | |
MedLine Citation:
|
PMID: 16144725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The enzyme telomerase plays a crucial role in cellular proliferation. By adding hexameric repeats to the chromosome ends, it prevents telomeric loss and, thus entry into senescence of limited life span cells. It is unclear, however, what would be the effect of over-expressing telomerase in an immortalised cell line, characterised by unlimited life span and high levels of apoptosis under sub-optimal growth conditions. In order to address this question, we have transfected the immortal cell line CHO K1 with the human telomerase reverse transcriptase (hTERT) catalytic subunit. Differences in the growth profile and apoptosis levels between the cells over-expressing hTERT (Telo) and the cells containing mock vector were found under standard growth conditions. Similarly, the Telo cells showed lower levels of apoptosis, greater attachment tendency and higher viable cell density under serum-deprived conditions compared to the control cell line, suggesting a major role for hTERT over-expression in stressed cultures. Using a mouse cDNA microarray, the collagen type III and V genes were shown to have at least a 10-fold higher expression in the Telo cells than the control cells, suggesting a role of hTERT in the cell attachment pathways. |
| | |
Authors:
|
Francesco Crea; Donatella Sarti; Francesco Falciani; Mohamed Al-Rubeai |
Related Documents
:
|
15034305 - The frequency of homologous recombination in human alt cells. 16404345 - Latent membrane protein-1 of epstein - barr virus increases sensitivity to arsenic trio... 9242615 - Bypass of senescence after disruption of p21cip1/waf1 gene in normal diploid human fibr... 20404145 - Esophageal cancer-related gene 4 is a secreted inducer of cell senescence expressed by ... 6692845 - Interaction of liposomes with kupffer cells in vitro. 7954875 - Fluorescence in the migrating pseudoplasmodium of the cellular slime mold dictyostelium... |
Publication Detail:
|
Type: Journal Article Date: 2005-09-06 |
Journal Detail:
|
Title: Journal of biotechnology Volume: 121 ISSN: 0168-1656 ISO Abbreviation: J. Biotechnol. Publication Date: 2006 Jan |
Date Detail:
|
Created Date: 2006-01-09 Completed Date: 2006-06-21 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8411927 Medline TA: J Biotechnol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 109-23 Citation Subset: IM |
Affiliation:
|
Department of Chemical Engineering, School of Engineering, University of Birmingham, Birmingham B15 2TT, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis / genetics* CHO Cells Catalytic Domain / genetics Cell Adhesion / genetics Cell Transformation, Neoplastic / genetics* Collagen Type III / biosynthesis Collagen Type V / biosynthesis Cricetinae Cricetulus DNA-Binding Proteins / biosynthesis*, genetics Gene Expression Regulation, Neoplastic / genetics Humans Recombinant Proteins / biosynthesis*, genetics Telomerase / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
|
0/Collagen Type III; 0/Collagen Type V; 0/DNA-Binding Proteins; 0/Recombinant Proteins; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Rubber additives induce oxidative stress in rainbow trout.
Next Document: Self-assembly of sensory neurons into ganglia-like microtissues.