| Over-expression of glucose dehydrogenase improves cell growth and riboflavin production in Bacillus subtilis. | |
| | |
MedLine Citation:
|
PMID: 16912926 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Ribulose 5-phosphate is a precursor for riboflavin biosynthesis. Alteration of carbon flow into the pentose phosphate pathway will affect the availability of ribulose 5-phosphate and the riboflavin yield. We have modulated carbon flow in Bacillus subtilis through the gluconate bypass by over-expression of glucose dehydrogenase under the control of the constitutively expressed P43 promoter. Over-expression of glucose dehydrogenase resulted in low acid production (acetate and pyruvate). The substantial reduction in acid production is accompanied by increased riboflavin production and an increased rate of growth while glucose consumption remained unchanged. Metabolic analysis indicated that over-expression of glucose dehydrogenase increased intracellular pool of ribulose 5-phosphate. The high concentrations of ribulose 5-phosphate could explain the increased riboflavin production. |
| | |
Authors:
|
Yingbo Zhu; Xun Chen; Tao Chen; Shuobo Shi; Xueming Zhao |
Related Documents
:
|
10798676 - Glucose-specific regulation of aldose reductase in human retinal pigment epithelial cel... 19067246 - Gtme for improved xylose fermentation of saccharomyces cerevisiae. 11090686 - Kinetics of glucose isomerization to fructose by immobilized glucose isomerase: anomeri... 8285716 - Alteration of growth yield by overexpression of phosphoenolpyruvate carboxylase and pho... 8202496 - Insulin promotes the biosynthesis and secretion of apolipoprotein b-48 by altering apol... 15273546 - The early effect of the roux-en-y gastric bypass on hormones involved in body weight re... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-08-16 |
Journal Detail:
|
Title: Biotechnology letters Volume: 28 ISSN: 0141-5492 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2006 Oct |
Date Detail:
|
Created Date: 2006-09-06 Completed Date: 2007-02-23 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 1667-72 Citation Subset: IM |
Affiliation:
|
Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, P. R. China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetic Acid
/
metabolism Bacillus subtilis / enzymology, growth & development, metabolism* Cloning, Molecular Glucose 1-Dehydrogenase / biosynthesis*, genetics Hydrogen-Ion Concentration Promoter Regions, Genetic Pyruvic Acid / metabolism Riboflavin / biosynthesis* |
| Chemical | |
Reg. No./Substance:
|
127-17-3/Pyruvic Acid; 64-19-7/Acetic Acid; 83-88-5/Riboflavin; EC 1.1.1.47/Glucose 1-Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecular cloning, stable expression and cellular localization of human alpha1-adrenergic receptor s...
Next Document: Expression of Helicobacter pylori urease subunit B gene in transgenic rice.