Document Detail


Over-expression of a cDNA for human ornithine decarboxylase in transgenic rice plants alters the polyamine pool in a tissue-specific manner.
MedLine Citation:
PMID:  11683273     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated how over-expression of a cDNA for human ornithine decarboxylase (odc) affects the polyamine pools in transgenic rice. We further investigated tissue-specific expression patterns and product accumulation levels of the transgene driven by either constitutive or seed-specific promoters. Our results indicate that: (1) whereas the expression of a heterologous arginine decarboxylase (adc) cDNA in rice resulted in increased putrescine and spermine levels only in seeds, plants engineered to express odc cDNA exhibited significant changes in the levels of all three major polyamines in seeds and also in vegetative tissues (leaves and roots); (2) there was no linear correlation between odc mRNA levels, ODC enzyme activity and polyamine accumulation, suggesting that control of the polyamine pathway in plants is more complex than in mammalian systems; (3) ODC activity and polyamine changes varied in different tissues, indicating that the pathway is regulated in a tissue-specific manner. Our results suggest that ODC rather than ADC is responsible for the regulation of putrescine synthesis in plants.
Authors:
O Lepri; L Bassie; G Safwat; P Thu-Hang; P Trung-Nghia; E Hölttä; P Christou; T Capell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular genetics and genomics : MGG     Volume:  266     ISSN:  1617-4615     ISO Abbreviation:  Mol. Genet. Genomics     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-30     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101093320     Medline TA:  Mol Genet Genomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  303-12     Citation Subset:  IM    
Affiliation:
Molecular Biotechnology Unit, John Innes Centre, Norwich Research Park, UK.
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MeSH Terms
Descriptor/Qualifier:
Carboxy-Lyases / genetics,  metabolism
Chromatography, Thin Layer
DNA, Complementary / genetics*
Gene Expression Regulation, Enzymologic
Humans
Ornithine Decarboxylase / genetics*
Oryza sativa / enzymology,  genetics*,  metabolism
Plant Leaves / enzymology,  genetics,  metabolism
Plant Roots / enzymology,  genetics,  metabolism
Plants, Genetically Modified / enzymology,  genetics*,  metabolism
Polyamines / metabolism*
RNA, Messenger / genetics,  metabolism
Seeds / enzymology,  genetics*,  metabolism
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Polyamines; 0/RNA, Messenger; EC 4.1.1.-/Carboxy-Lyases; EC 4.1.1.17/Ornithine Decarboxylase; EC 4.1.1.19/arginine decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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