| Over-expression of AtDREB1A in chrysanthemum enhances tolerance to heat stress. | |
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MedLine Citation:
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PMID: 19234675 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of over-expression of AtDREB1A gene in chrysanthemums on heat stress tolerance was investigated. Transgenic plants with 35S:AtDREB1A construct (henceforth, 35S plants) and wild type (WT) plants were exposed to 45 degrees C as a heat stress treatment. When heat-treated for 36 h and followed by a 3-week recovery, approximately 70% of the 35S plants, but less than 20% of the WT plants were survived. Leaf electrolyte leakage was significantly lower in 35S plants than in WT plants. cDNA macroarray analysis showed that 55 of 74 DREB1A regulon members were up-regulated under heat stress in WT plants. In comparison with WT plants, 35S plants displayed greatly enhanced expression of the genes including signal transduction, transcription and HSP70 in the early time, and the genes including photosynthesis and metabolism in the late time of the heat treatment. We also found that the 35S plants maintained significantly higher photosynthetic capacity, and elevated activity of Rubisco and sucrose-phosphate synthase under heat stress. These results suggest that improvement of heat stress tolerance in transgenic chrysanthemum may be associated with enhanced tolerance of photosynthesis. |
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Authors:
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Bo Hong; Chao Ma; Yingjie Yang; Ting Wang; Kazuko Yamaguchi-Shinozaki; Junping Gao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-21 |
Journal Detail:
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Title: Plant molecular biology Volume: 70 ISSN: 1573-5028 ISO Abbreviation: Plant Mol. Biol. Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-05 Completed Date: 2009-06-02 Revised Date: 2009-06-26 |
Medline Journal Info:
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Nlm Unique ID: 9106343 Medline TA: Plant Mol Biol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 231-40 Citation Subset: IM |
Affiliation:
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College of Landscape and Architecture, Northeast Forestry University, Harbin, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis Proteins
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genetics* Chrysanthemum / genetics, metabolism* Gene Expression Profiling Gene Expression Regulation, Plant Glucosyltransferases / metabolism Hot Temperature* Photosynthesis Plant Leaves / genetics, metabolism Plants, Genetically Modified / genetics, metabolism RNA, Plant / metabolism Ribulose-Bisphosphate Carboxylase / metabolism Signal Transduction Stress, Physiological* Transcription Factors / genetics* Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/DREB1A protein, Arabidopsis; 0/RNA, Plant; 0/Transcription Factors; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.14/sucrose-phosphate synthase; EC 4.1.1.39/Ribulose-Bisphosphate Carboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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