Document Detail


The over-expression of an Arabidopsis B3 transcription factor, ABS2/NGAL1, leads to the loss of flower petals.
MedLine Citation:
PMID:  23185464     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transcriptional regulations are involved in many aspects of plant development and are mainly achieved through the actions of transcription factors (TF). To investigate the mechanisms of plant development, we carried out genetic screens for mutants with abnormal shoot development. Taking an activation tagging approach, we isolated a gain-of-function mutant abs2-1D (abnormal shoot 2-1D). abs2-1D showed pleiotropic growth defects at both the vegetative and reproductive developmental stages. We cloned ABS2 and it encodes a RAV sub-family of plant B3 type of transcriptional factors. Phylogenetic analysis showed that ABS2 was closely related to NGATHA (NGA) genes that are involved in flower development and was previously named NGATHA-Like 1 (NGAL1). NGAL1 was expressed mainly in the root and the filament of the stamen in flower tissues and sub-cellular localization assay revealed that NGAL1 accumulated in the nucleus. Interestingly, over-expression of NGAL1 driven by the constitutive 35S promoter led to transgenic plants with conspicuous flower defects, particularly a loss-of-petal phenotype. A loss-of-function ngal1-1 mutant did not show obvious phenotype, suggesting the existence of redundant activities and also the utility of gain-of-function genetic screens. Our results show that the over-expression of NGAL1 is capable of altering flower petal development, as well as shoot development.
Authors:
Jingxia Shao; Xiayan Liu; Rui Wang; Gaisheng Zhang; Fei Yu
Related Documents :
23969414 - One antitoxin--two functions: sr4 controls toxin mrna decay and translation.
24376004 - The ancient small gtpase rab21 functions in intermediate endocytic steps in trypanosomes.
2120074 - Expression of t cell receptor genes in the thymus: localization of transcripts in situ ...
8041754 - Major changes in the expression of the mrnas for cholinergic differentiation factor/leu...
24237754 - Management of the er stress by activation of the heat shock response in yeast.
9328214 - The androgen-dependent mouse seminal vesicle secretory protein of 99 amino acids (msvsp...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-21
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-11-27     Completed Date:  2013-05-30     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e49861     Citation Subset:  IM    
Affiliation:
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Arabidopsis* / genetics,  growth & development,  metabolism
Arabidopsis Proteins / genetics,  metabolism*
Flowers* / genetics,  growth & development,  metabolism
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Mutation
Phenotype
Phylogeny
Plant Roots* / growth & development,  metabolism
Plant Shoots / genetics,  growth & development*
Plants, Genetically Modified
Transcription Factors / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/ABS2 protein, Arabidopsis; 0/Arabidopsis Proteins; 0/NGA1 protein, Arabidopsis thaliana; 0/Transcription Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Influenza A(H1N1)pdm09 virus suppresses RIG-I initiated innate antiviral responses in the human lung...
Next Document:  Orai1 mediates exacerbated Ca(2+) entry in dystrophic skeletal muscle.