Document Detail

Ovariectomy fails to modify the cardiac myosin isoenzyme profile of adult rats.
MedLine Citation:
PMID:  9543690     Owner:  NLM     Status:  MEDLINE    
Estrogen has been shown to help maintain the elevated expression of the high ATPase myosin isoform, V1, present in the hearts of young rats (< 70 days of age). Because hearts of this age are still undergoing significant maturation, the current study sought to determine if estrogen similarly regulates myosin isoenzyme expression in the mature adult heart. To make this determination, ten month old retired female Sprague-Dawley rats were made estrogen-deficient by ovariectomy (OVAR, n = 8). Sham-operated (CONTR, n = 8) animals served as controls. Nine weeks later, the animals were sacrificed and left ventricular tissue collected. Crude myofibrills were isolated from these samples and electrophoretically separated into the three isoenzymatic forms of cardiac myosin (V1, V2, and V3). OVAR animals were larger than the CONTR group (p < 0.05), but heart weight/body weight ratios were not different between groups. Distribution of myosin among its three isoenzymes was similar between groups (CONTR: V1, 80%, V2, 14%; V3, 6%; OVAR: V1, 77%, V2, 16%, V3, 7%). These data demonstrate that myosin isoenzyme distribution in the adult heart is unaltered by ovariectomy, suggesting that estrogen loses its ability to regulate expression of this protein in the mature heart.
G S Morris; S A Melton; M Hegsted; W P Bartlett
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme     Volume:  30     ISSN:  0018-5043     ISO Abbreviation:  Horm. Metab. Res.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-06-01     Completed Date:  1998-06-01     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0177722     Medline TA:  Horm Metab Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  84-7     Citation Subset:  IM    
School of Physical Therapy, Texas Woman's University, Houston, USA.
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MeSH Terms
Body Weight / physiology
Estrogens / physiology
Gene Expression Regulation, Developmental / physiology
Heart Ventricles / growth & development
Isoenzymes / metabolism
Myocardium / enzymology*
Myosins / metabolism*
Organ Size / physiology
Rats, Sprague-Dawley
Reg. No./Substance:
0/Estrogens; 0/Isoenzymes; EC

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