| Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration. | |
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MedLine Citation:
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PMID: 22710371 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Estrogens are a family of female sexual hormones with an exceptionally wide spectrum of effects. When rats and mice are used in estrogen research they are commonly ovariectomized in order to ablate the rapidly cycling hormone production, replacing the 17β-estradiol exogenously. There is, however, lack of consensus regarding how the hormone should be administered to obtain physiological serum concentrations. This is crucial since the 17β-estradiol level/administration method profoundly influences the experimental results(1-3). We have in a series of studies characterized the different modes of 17β-estradiol administration, finding that subcutaneous silastic capsules and per-oral nut-cream Nutella are superior to commercially available slow-release pellets (produced by the company Innovative Research of America) and daily injections in terms of producing physiological serum concentrations of 17β-estradiol(4-6). Amongst the advantages of the nut-cream method, that previously has been used for buprenorphine administration(7), is that when used for estrogen administration it resembles peroral hormone replacement therapy and is non-invasive. The subcutaneous silastic capsules are convenient and produce the most stable serum concentrations. This video article contains step-by-step demonstrations of ovariectomy and 17β-estradiol hormone replacement by silastic capsules and peroral Nutella in rats and mice, followed by a discussion of important aspects of the administration procedures. |
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Authors:
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Jakob O Ström; Annette Theodorsson; Edvin Ingberg; Ida-Maria Isaksson; Elvar Theodorsson |
Publication Detail:
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Type: Journal Article Date: 2012-06-07 |
Journal Detail:
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Title: Journal of visualized experiments : JoVE Volume: - ISSN: 1940-087X ISO Abbreviation: J Vis Exp Publication Date: 2012 |
Date Detail:
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Created Date: 2012-06-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101313252 Medline TA: J Vis Exp Country: United States |
Other Details:
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Languages: eng Pagination: - Citation Subset: IM |
Affiliation:
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Clinical Chemistry and Neurosurgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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