Document Detail


Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration.
MedLine Citation:
PMID:  22710371     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Estrogens are a family of female sexual hormones with an exceptionally wide spectrum of effects. When rats and mice are used in estrogen research they are commonly ovariectomized in order to ablate the rapidly cycling hormone production, replacing the 17β-estradiol exogenously. There is, however, lack of consensus regarding how the hormone should be administered to obtain physiological serum concentrations. This is crucial since the 17β-estradiol level/administration method profoundly influences the experimental results(1-3). We have in a series of studies characterized the different modes of 17β-estradiol administration, finding that subcutaneous silastic capsules and per-oral nut-cream Nutella are superior to commercially available slow-release pellets (produced by the company Innovative Research of America) and daily injections in terms of producing physiological serum concentrations of 17β-estradiol(4-6). Amongst the advantages of the nut-cream method, that previously has been used for buprenorphine administration(7), is that when used for estrogen administration it resembles peroral hormone replacement therapy and is non-invasive. The subcutaneous silastic capsules are convenient and produce the most stable serum concentrations. This video article contains step-by-step demonstrations of ovariectomy and 17β-estradiol hormone replacement by silastic capsules and peroral Nutella in rats and mice, followed by a discussion of important aspects of the administration procedures.
Authors:
Jakob O Ström; Annette Theodorsson; Edvin Ingberg; Ida-Maria Isaksson; Elvar Theodorsson
Publication Detail:
Type:  Journal Article     Date:  2012-06-07
Journal Detail:
Title:  Journal of visualized experiments : JoVE     Volume:  -     ISSN:  1940-087X     ISO Abbreviation:  J Vis Exp     Publication Date:  2012  
Date Detail:
Created Date:  2012-06-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101313252     Medline TA:  J Vis Exp     Country:  United States    
Other Details:
Languages:  eng     Pagination:  -     Citation Subset:  IM    
Affiliation:
Clinical Chemistry and Neurosurgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University.
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