Document Detail


Ovarian ultrastructure in virgin queens of Apis mellifera L. narcotized by CO(2).
MedLine Citation:
PMID:  22421335     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Ovaries of newly emerged virgin queens, aged 1, 3, 5, and 10 days, were submitted to an atmosphere of CO(2) for 1min, and studied by transmission electron microscopy. Newly emerged control and experimental queens were kept caged during 15 days with six nurse workers (changed every 2 days) and fed with sugar candy and water ad libitum. For the ultrastructural studies, the ovaries were collected when the queens were 3, 5, 10, and 15 days old. The effect of the treatment on the ovary was mainly evaluated by cell death incidence and acid phosphatase detection. Neither the confinement nor CO(2) treatment interfered with the pattern of queen ovarian development for up to 5 days; that is, no differences were observed in ovarian ultrastructure or acid phosphatase activity between control and treated queens. Beyond the age of 10 days, treated queens showed a lower rate of cell death than controls, but the positivity to acid phosphatase reaction remained similar in both. The protective effect of CO(2) on cells, however, did not persist, and 15-day-old treated queens showed no differences compared to the controls in the rates of cell death or enzyme reactivity. In conclusion, treatment with CO(2) did not significantly change the ultrastructure of ovarian cells, or acid phosphatase activity in them, during the caging time of the queens. From an applied perspective, the short-term imprisonment of newly emerged queens outside of the colony, as is done by beekeepers, does not seem to affect their performance in terms of future fertility, although the narcosis, as applied in this study, may negatively affect their long-term performance.
Authors:
Bruno Berger; Carminda da Cruz-Landim
Related Documents :
16366675 - Sweet and sour cherry phenolics and their protective effects on neuronal cells.
17533865 - Transformation of polycyclic aromatic hydrocarbons by laccase is strongly enhanced by p...
23353655 - Sonhafouonic acid, a new cytotoxic and antifungal hopene-triterpenoid from zheneria sca...
2285225 - Stimulation of granulocytic cell iodination by tannins and related compounds.
15575755 - Preparation of fluoroalkyl imines, amines, enamines, ketones, alpha-amino carbonyls, an...
2837825 - Probing the mechanisms of macromolecular recognition: the cytochrome b5-cytochrome c co...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-23
Journal Detail:
Title:  Micron (Oxford, England : 1993)     Volume:  -     ISSN:  1878-4291     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-3-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312850     Medline TA:  Micron     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Departamento de Biologia, Instituto de Biociências de Rio Claro, Universidade Estadual Paulista (UNESP), Av. 24 A, 1515, Bela Vista, 13.506-900 Rio Claro, São Paulo, Brazil.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Compliance profile of the human cornea as measured by atomic force microscopy.
Next Document:  Interleukin-6 receptor pathways in coronary heart disease: a collaborative meta-analysis of 82 studi...