Document Detail


Ovarian superstimulation, transrectal ultrasound-guided oocyte recovery, and IVF in rhinoceros.
MedLine Citation:
PMID:  19720394     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Numerous reports on reproductive pathology in all rhinoceros species illustrate the abundance of female infertility in captive populations. In infertile rhinoceroses, oocyte collection and embryo production could represent the best remaining option for these animals to reproduce and to contribute to the genetic pool. We report here on superstimulation, repeated oocyte recovery, and attempted in vitro fertilization (IVF) in white and black rhinoceroses. Four anestrous rhinoceroses (two white, two black) with unknown follicular status were treated with gonadotropin-releasing hormone analogue, deslorelin acetate, for 6 to 7 d. Number and size of follicles in superstimulated females was significantly higher and larger compared with those in nonstimulated anestrous females (n=9). Ovum pick-up was achieved by transrectal ultrasound-guided follicle aspiration. Up to 15 follicles were aspirated per ovary. During six ovum pick-ups, a total of 29 cumulus-oocyte complexes (COCs) were harvested with a range of 2 to 9 COCs per collection. No postsurgical complications were noted on the rhinoceros ovaries using this minimally invasive approach. Various in vitro maturation (IVM) and IVF protocols were tested on the collected COCs. Despite the low total number of COCs available for IVM and IVF in this study, we can report the first rhinoceros embryo ever produced in vitro. The production of a 4-cell embryo demonstrated the potential of transrectal ultrasound-guided oocyte recovery as a valuable tool for in vitro production of rhinoceros embryos from otherwise infertile females.
Authors:
R Hermes; F Göritz; T J Portas; B R Bryant; J M Kelly; L J Maclellan; T Keeley; F Schwarzenberger; C Walzer; A Schnorrenberg; R E Spindler; J Saragusty; S Kaandorp; T B Hildebrandt
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Publication Detail:
Type:  Journal Article     Date:  2009-08-31
Journal Detail:
Title:  Theriogenology     Volume:  72     ISSN:  1879-3231     ISO Abbreviation:  Theriogenology     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-21     Completed Date:  2009-12-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  959-68     Citation Subset:  IM    
Affiliation:
Leibniz Institute for Zoo and Wildlife Research, PF 601103, Berlin, Germany. hermes@izw-berlin.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Fertilization in Vitro / veterinary*
Infertility, Female / therapy,  veterinary*
Male
Oocytes*
Ovary / ultrasonography
Perissodactyla*
Sperm Injections, Intracytoplasmic / veterinary
Suction / veterinary
Superovulation*
Tissue and Organ Harvesting / methods,  veterinary*
Ultrasonography / veterinary

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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