Document Detail

Ovarian and hemolymph ecdysteroid titers during vitellogenesis in Macrobrachium rosenbergii.
MedLine Citation:
PMID:  8319875     Owner:  NLM     Status:  MEDLINE    
Changes in ovarian and hemolymph ecdysteroid concentration and composition during vitellogenesis have been investigated in the freshwater prawn Macrobrachium rosenbergii. Free ecdysteroids (20-hydroxyecdysone and ecdysone) in hemolymph increased in concentration during vitellogenesis from zero at stage 0 to 1.5 ng/ml at stage I to 7.3 ng/ml in mature, stage IV animals. 20-Hydroxyecdysonoic acid (1.2 ng/ml) was detected in the stage IV hemolymph. Ovarian-free ecdysteroid concentration, expressed as nanograms per gram of tissue, fell during vitellogenesis from 83.2 ng/g at stage 0, non-pigmented tissue to 14.2 ng/g at stage IV tissue, being minimal at stage I (6.3 ng/g). However, expression of ovarian free ecdysteroid content as nanograms per ovary revealed a rise from 7.7 ng/ovary at stage 0, nonpigmented tissue to 28.3 ng/ovary at stage IV, again being minimal at stage I (2.0 ng/ovary). 20-Hydroxyecdysonoic acid and ecdysonoic acid were identified at ovarian stages II-III and stage IV.
N J Young; S G Webster; H H Rees
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  General and comparative endocrinology     Volume:  90     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-08-05     Completed Date:  1993-08-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  183-91     Citation Subset:  IM    
Department of Biochemistry, University of Liverpool, UK.
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MeSH Terms
Chromatography, High Pressure Liquid
Gas Chromatography-Mass Spectrometry
Hemolymph / chemistry,  metabolism*
Invertebrate Hormones / isolation & purification,  metabolism*
Ovary / chemistry,  metabolism*
Palaemonidae / metabolism*
Steroids / isolation & purification,  metabolism*
Vitellogenesis / physiology*
Reg. No./Substance:
0/Ecdysteroids; 0/Invertebrate Hormones; 0/Steroids

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