Document Detail


Ovarian follicle apoptosis at the onset of standing estrus in virgin and repeat-breeder dairy heifers.
MedLine Citation:
PMID:  11665874     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is evidence that repeat breeding in dairy cattle can be caused by both extrinsic, environmental factors and intrinsic, animal factors. In repeat-breeder heifers (RBH), disturbed endocrine patterns and estrous events result in a subsequent decreased fertility associated with delayed ovulation. Whether infertility is also due to the presence of an unsuitable follicular environment impairing normal fertilization, remains to be determined. At the onset of standing estrus, ovaries were obtained from 7 strictly defined RBH and 5 virgin heifers (VH) of the Swedish Red and White breed. Detection of apoptosis in the preovulatory and three subordinate follicle walls was done by using the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) technique at light microscopy level. The follicles were histologically assessed for degree of atresia. The ultrastructure of the follicle wall and recovered oocytes was studied using transmission electron microscopy. The overall degree of apoptosis in membrana granulosa and theca interna of preovulatory and subordinate follicles did not differ between RBH and VH, but the numbers of TUNEL-positive cells differed significantly between preovulatory and subordinate follicles in both RBH and VH. There was a strong relationship between density of apoptotic cells and degree of atresia. No differences in follicle wall apoptosis nor morphology were detectable, suggesting that repeat breeder heifers enter standing estrus with the same morphological prerequisites as normal animals, considering follicular structure.
Authors:
R Båge; W T Bosu; H Rodríguez-Martínez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Theriogenology     Volume:  56     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-10-22     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  699-712     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala. renee.bage@og.slu.se
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Breeding
Cattle
Estrus*
Female
Follicular Atresia
In Situ Nick-End Labeling
Microscopy, Electron
Ovarian Follicle / ultrastructure*
Ovariectomy
Ovary / ultrastructure
Ovulation

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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