Document Detail

Ovalbumin increases macromolecular efflux from the in situ nasal mucosa of allergic hamsters.
MedLine Citation:
PMID:  9451632     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to determine whether bradykinin mediates ovalbumin-induced increase in macromolecular efflux from the nasal mucosa of ovalbumin-sensitized hamsters in vivo and, if so, whether the L-arginine/nitric oxide biosynthetic pathway transduces, in part, this response. We found that suffusion of ovalbumin onto the in situ nasal mucosa of ovalbumin-sensitized hamsters, but not of controls, elicited a significant time- and concentration-dependent increase in clearance of fluorescein isothiocyanate-labeled dextran (mol mass, 70 kDa; P < 0.05). HOE-140, but not des-Arg9,[Leu8]-bradykinin, and NG-L-arginine methyl ester (L-NAME), but not NG-D-arginine methyl ester, significantly attenuated ovalbumin-induced responses. L-Arginine, but not D-arginine, abolished the effects of L-NAME. L-NAME also significantly attenuated bradykinin-, but not adenosine-induced increase in macromolecular efflux from the in situ nasal mucosa. Overall, these data suggest that ovalbumin increases macromolecular efflux from the in situ nasal mucosa of ovalbumin-sensitized hamsters, in part, by producing bradykinin with subsequent activation of the L-arginine/ nitric oxide biosynthetic pathway.
X P Gao; S R Akhter; I Rubinstein
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  84     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-03-20     Completed Date:  1998-03-20     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  169-76     Citation Subset:  IM    
Department of Medicine, University of Illinois at Chicago, USA.
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MeSH Terms
Adenosine / pharmacology
Arginine / metabolism
Bradykinin / pharmacology
Enzyme Inhibitors / pharmacology
Exudates and Transudates / metabolism,  physiology
Fluorescein-5-isothiocyanate / analogs & derivatives
NG-Nitroarginine Methyl Ester / pharmacology
Nasal Decongestants / pharmacology
Nasal Mucosa / metabolism*
Nitric Oxide / metabolism
Nitric Oxide Synthase / antagonists & inhibitors
Ovalbumin / metabolism,  pharmacology*
Phenylephrine / pharmacology
Receptors, Bradykinin / antagonists & inhibitors
Respiratory Hypersensitivity / metabolism*
Serine Proteinase Inhibitors / metabolism*
Vasodilator Agents / pharmacology
Grant Support
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Nasal Decongestants; 0/Receptors, Bradykinin; 0/Serine Proteinase Inhibitors; 0/Vasodilator Agents; 0/fluorescein isothiocyanate dextran; 10102-43-9/Nitric Oxide; 3326-32-7/Fluorescein-5-isothiocyanate; 50903-99-6/NG-Nitroarginine Methyl Ester; 58-61-7/Adenosine; 58-82-2/Bradykinin; 59-42-7/Phenylephrine; 74-79-3/Arginine; 9004-54-0/Dextrans; 9006-59-1/Ovalbumin; EC Oxide Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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