Document Detail

Outflow facility studies in the perfused human ocular anterior segment.
MedLine Citation:
PMID:  1855546     Owner:  NLM     Status:  MEDLINE    
We have recently developed a tissue model of the human aqueous outflow pathway involving placement of the eviscerated anterior corneoscleral shell, [with lens and uveal tissue removed but trabecular meshwork (TM) attached] onto a specialized perfusion apparatus. The TM and associated outflow tissues are perfused with culture medium at a physiologically-relevant perfusion pressure in a 5% CO2 environment at 37 degrees C. Under these conditions, the perfused outflow tissues are similar for several days, to the human and/or subhuman primate outflow system in vivo with regard to morphology as well as several functional parameters. Measured facility of outflow (0.271 +/- 0.018 microliters min-1 mmHg-1, n = 79) is similar to facility values obtained by tonography in living human beings. Moreover, outflow facility decreases in a linear fashion with increased perfusion pressure by 1.4% mmHg-1. Finally the removal of the TM results in a 41% decrease in measured outflow resistance. The ability to study viable human outflow tissue for at least several days and the opportunity to establish a model which serves as an alternative to animal testing, point to the potential importance of this technique in investigating the biology of the aqueous outflow system.
K Erickson-Lamy; J W Rohen; W M Grant
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental eye research     Volume:  52     ISSN:  0014-4835     ISO Abbreviation:  Exp. Eye Res.     Publication Date:  1991 Jun 
Date Detail:
Created Date:  1991-08-29     Completed Date:  1991-08-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370707     Medline TA:  Exp Eye Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  723-31     Citation Subset:  IM    
Howe Laboratory of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston 02114.
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MeSH Terms
Anterior Eye Segment / physiology*,  ultrastructure
Aqueous Humor / physiology*
Microscopy, Electron
Middle Aged
Models, Biological
Organ Culture Techniques
Perfusion / instrumentation
Trabecular Meshwork / physiology,  ultrastructure
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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