| An outer arm Dynein conformational switch is required for metachronal synchrony of motile cilia in planaria. | |
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MedLine Citation:
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PMID: 20844081 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Motile cilia mediate the flow of mucus and other fluids across the surface of specialized epithelia in metazoans. Efficient clearance of peri-ciliary fluids depends on the precise coordination of ciliary beating to produce metachronal waves. The role of individual dynein motors and the mechanical feedback mechanisms required for this process are not well understood. Here we used the ciliated epithelium of the planarian Schmidtea mediterranea to dissect the role of outer arm dynein motors in the metachronal synchrony of motile cilia. We demonstrate that animals that completely lack outer dynein arms display a significant decline in beat frequency and an inability of cilia to coordinate their oscillations and form metachronal waves. Furthermore, lack of a key mechanosensitive regulatory component (LC1) yields a similar phenotype even though outer arms still assemble in the axoneme. The lack of metachrony was not due simply to a decrease in ciliary beat frequency, as reducing this parameter by altering medium viscosity did not affect ciliary coordination. In addition, we did not observe a significant temporal variability in the beat cycle of impaired cilia. We propose that this conformational switch provides a mechanical feedback system within outer arm dynein that is necessary to entrain metachronal synchrony. |
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Authors:
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Panteleimon Rompolas; Ramila S Patel-King; Stephen M King |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-15 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 21 ISSN: 1939-4586 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-29 Completed Date: 2011-02-22 Revised Date: 2011-07-20 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 3669-79 Citation Subset: IM |
Affiliation:
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Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030-3305, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium Signaling Cilia / enzymology, physiology, ultrastructure Dyneins / deficiency, physiology* Epithelium / physiology Locomotion / physiology Mechanoreceptors / physiology Models, Animal Planarians / cytology, genetics, physiology*, ultrastructure Protein Conformation |
| Grant Support | |
ID/Acronym/Agency:
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GM51293/GM/NIGMS NIH HHS; R01 GM051293-15/GM/NIGMS NIH HHS; R01 GM051293-16/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.6.4.2/Dyneins |
| Comments/Corrections | |
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