Document Detail

Outcome of ICSI using fresh and cryopreserved-thawed testicular spermatozoa in patients with non-mosaic Klinefelter's syndrome.
MedLine Citation:
PMID:  11726584     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Recently, intracytoplasmic sperm injection (ICSI) of testicular spermatozoa retrieved surgically from patients with non-mosaic Klinefelter's syndrome resulted in several deliveries. The aim of this study was to evaluate the outcome of ICSI using fresh and cryopreserved-thawed testicular spermatozoa in these patients. METHODS AND RESULTS: Following informed consent regarding the genetic risks of their potential offspring, mature testicular spermatozoa were found in five out of 12 (42%) patients who underwent testicular sperm extraction, and ICSI was performed while excess tissue was cryopreserved. The mean age of the patients was 28.7 +/- 3.6 (range 23-36 years). Their baseline FSH was elevated (mean 38.3 +/- 11.4; range 22-58 mIU/ml). All patients had small testicles of 2-4 ml in volume. The outcome of ICSI using fresh or cryopreserved-thawed testicular spermatozoa during five cycles in each group, was compared. No statistical significant difference was found in the two pronuclear fertilization rate (66 versus 58%), embryo cleavage rate (98 versus 90%) and embryo implantation rate (33.3 versus 21.4%) for fresh or cryopreserved sperm accordingly. The clinical outcome after using fresh testicular sperm included two singleton pregnancies (one delivered and one ongoing) and a triplet pregnancy resulting in a twin delivery (after reduction of an 47,XXY embryo). After using cryopreserved-thawed testicular spermatozoa, two pregnancies were obtained resulting in one delivery of twins and one early spontaneous abortion. CONCLUSIONS: Outcome of ICSI using cryopreserved-thawed testicular spermatozoa of patients with non-mosaic Klinefelter's syndrome is comparable with that following the use of fresh spermatozoa. The genetic implications for the future offspring should be explained to the patients.
S Friedler; A Raziel; D Strassburger; M Schachter; O Bern; R Ron-El
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  16     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-11-29     Completed Date:  2002-02-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  2616-20     Citation Subset:  IM    
IVF and Infertility Unit, Assaf Harofeh Medical Center, Tel Aviv University, Israel.
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MeSH Terms
Cleavage Stage, Ovum
Embryo Transfer
Embryo, Mammalian / physiology
Follicle Stimulating Hormone / blood
Infertility, Male / etiology,  therapy
Klinefelter Syndrome / complications*
Pregnancy Outcome
Sperm Injections, Intracytoplasmic*
Spermatozoa / physiology*
Testis / cytology*
Testosterone / blood
Treatment Outcome*
Reg. No./Substance:
58-22-0/Testosterone; 9002-68-0/Follicle Stimulating Hormone

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