Document Detail


Ouabain sensitivity is linked to ras -transformation in human HOS cells.
MedLine Citation:
PMID:  3458466     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mouse cells transformed by the retroviral oncogene v-Ki- ras are significantly more sensitive to the toxic effects of 1mM ouabain than are their nontransformed counterparts. We have extended these findings to a human cell line (HOS). HOS cells (ATCC CRL 1543) are relatively resistant to treatment with 1 microM ouabain while KHOS cells (transformed by Kirsten murine sarcoma virus) are extremely sensitive. Two flat revertant cell lines isolated from the KHOS line and lacking the v- ras gene sequences are resistant to ouabain. This effect may be observed morphologically and can also be demonstrated by dye exclusion and plating efficiency tests. In addition, the toxic effects of ouabain may be rapidly and efficiently quantitated in a 51Cr-release assay. This differential lethality may be used to enrich the proportion of non-transformed revertants in populations of mutagen-treated transformed cells.
Authors:
L E Benade; N Talbot; P Tagliaferri; C Hardy; J Card; M Noda; N Najam; R H Bassin
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  136     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-06-13     Completed Date:  1986-06-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  807-14     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Survival / drug effects
Cell Transformation, Neoplastic*
Cell Transformation, Viral*
Chromium Radioisotopes
Drug Resistance
Humans
Kirsten murine sarcoma virus
Mice
Oncogenes*
Osteosarcoma
Ouabain / pharmacology*
Chemical
Reg. No./Substance:
0/Chromium Radioisotopes; 630-60-4/Ouabain

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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