Document Detail

Ouabain-like compound changes rapidly on physical exercise in humans and dogs: effects of beta-blockade and angiotensin-converting enzyme inhibition.
MedLine Citation:
PMID:  15837822     Owner:  NLM     Status:  MEDLINE    
Ouabain, an inhibitor of the sodium pump, has been identified as a constituent of bovine adrenal glands. We were interested whether the release of this cardiotonic steroid is stimulated by physical exercise. Hence, athletes and healthy dogs were subjected to ergometry. Ouabain-like compound (OLC) was measured in venous blood by enzyme-linked immunosorbent assay as well as by (86)Rb+ uptake inhibition (as ouabain equivalents). OLC increased in venous blood of athletes after 15 minutes of ergometry from 2.5+/-0.5 to 86.0+/-27.2 nmol/L (n=51; P<0.001), as did the concentration of a circulating inhibitor of the sodium pump from 7.3+/-1.7 to 129.8+/-51 nmol/L (ouabain equivalents, P<0.05). Half-maximal increase in heart rate and systolic blood pressure occurred at 5.1+/-1.2 nmol/L and at 30+/-1 nmol/L OLC, respectively. On rest, OLC decreased in humans and dogs with a half-life of 3 to 5 minutes. In beagles exposed to moderate exercise on a treadmill for 13 minutes, levels of OLC increased 46-fold (from 3.7+/-0.8 to 166.9+/-91.8 nmol/L; n=6; P<0.005). This effect was suppressed when the dogs had been treated for 3 weeks with the beta1-adrenergic receptor blocker atenolol or the angiotensin-converting enzyme inhibitor benazepril. We conclude that OLC changes rapidly during exercise and is under the control of norepinephrine and angiotensin II.
Natali Bauer; Jochen Müller-Ehmsen; Ulrike Krämer; Njde Hambarchian; Carsten Zobel; Robert H G Schwinger; Horst Neu; Ulrike Kirch; Ernst-Günther Grünbaum; Wilhelm Schoner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-04-18
Journal Detail:
Title:  Hypertension     Volume:  45     ISSN:  1524-4563     ISO Abbreviation:  Hypertension     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-04-29     Completed Date:  2005-10-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1024-8     Citation Subset:  IM    
Clinic for Small Animal Internal Medicine and Forensic Affairs, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Germany.
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MeSH Terms
Adrenergic beta-Antagonists / pharmacology*
Angiotensin-Converting Enzyme Inhibitors / pharmacology*
Atenolol / pharmacology
Benzazepines / pharmacology
Cardenolides / blood*
Enzyme-Linked Immunosorbent Assay
Exercise / physiology*
Exercise Test
Motor Activity / physiology*
Ouabain / blood
Saponins / blood*
Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors
Reg. No./Substance:
0/Adrenergic beta-Antagonists; 0/Angiotensin-Converting Enzyme Inhibitors; 0/Benzazepines; 0/Cardenolides; 0/Saponins; 0/digoxin-like factors; 29122-68-7/Atenolol; 630-60-4/Ouabain; 86541-75-5/benazepril; EC ATPase
Comment In:
Hypertension. 2005 Oct;46(4):e13; author reply e13   [PMID:  16172419 ]
Hypertension. 2005 Sep;46(3):e9; author reply e9-10   [PMID:  16103262 ]

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